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We have employed whole genome microarray expression profiling to identify genes differentially expressed in neonatal bowel tissues of different gastrointestinal conditions. Bowel tissues were collected from infants who underwent surgery treatment for necrotising enterocolitis (NEC) and spontaneous i...
ORGANISM(S): Homo sapiens 
Synchronized C. elegans cultures were prepared using standard techniques (http://cmgm.stanford.edu/~kimlab/index_methods.html). Live young adult worms were split between NG plates pre-seeded with the non-pathogenic E. coli strain OP50 or the Pseudomonas aeruginosa clinical isolate PA14 and incubated...
ORGANISM(S): Caenorhabditis elegans 
We have employed miRNA microarray expression profiling to identify miRNA differentially expressed in neonatal bowel tissues with different gastrointestinal conditions. Bowel tissues were collected from infants who underwent surgery treatment for necrotizing enterocolitis (NEC), spontaneous intestina...
ORGANISM(S): Homo sapiens 
Endogenous bioelectric signaling via changes in cellular resting potential (Vmem) is a key regulator of patterning during regeneration and embryogenesis in numerous model systems. Depolarization of Vmem has been functionally implicated in de-differentiation, tumorigenesis, anatomical re-specificatio...
ORGANISM(S): Homo sapiens 
We used microarrays to determine if miRNA expression in human airway smooth muscle cells is altered by a pro-inflammatory stimulus. A majority of the miRNAs on the array exhibited very low signal intensity. In ASM cells exposed to IL-1β, TNFα, and IFNγ, none of the miRNA expressed were signific...
ORGANISM(S): Homo sapiens 
We developed a novel approach, m6A-seq, for high-resolution mapping of the transcriptome-wide m6A landscape, based on antibody-mediated capture followed by massively parallel sequencing. Identification of m6A modified sequences in HepG2 cells. HepG2 cells were incubated with either IFNg (200ng/ml) o...
ORGANISM(S): Homo sapiens 
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