To support the development of a novel bioinformatic solution for glycopeptide identification, the cancer-associated AXL glycoprotein was used to validate the approach. AXL has 6 N-glycan sites.
The glycosylation of antibody-based proteins is vital in conferring the right therapeutic outcomes of the patient. Despite this, significant infrastructure is required to analyse biologic glycosylation in various unit operations from biologic development, process development to QA/QC in bio-manufact...
Supplementary information for a technical note describing the application of cysteine aminoethylation for the site-specific glycosylation analysis of recombinant human EPO using trypsin as sole protease.
