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ProteomicsDIA-PASEF analysis of lung tissues from influenza-infected and uninfected mice
Lung tissues from uninfected and infected mice (n = 5 per group) were homogenized in 0.5 mL of guanidine buffer. After heating and sonication, the lysates were centrifuged at 20,000 g for 15 min at 4° C. The resulting supernatants were collected, and the proteins (20 µg each) were purified using the...
ORGANISM(S): Mus Musculus (mouse) 
2026-01-31 | PXD069450 | JPOST Repository
ProteomicsLFQ of neo-amino terminal peptides using Tryp-N
To determine candidate OMA1 substrates during mitochondrial stress, OMA1 KO MEFs or its rescued cells expressing OMA1-Myc were treated with or without 40 uM FCCP for 2 h, then washed once with 1× PBS (pH 7.4), and cell pellets were collected. The pellets were then lysed in a guanidine buffer [6 M gu...
ORGANISM(S): Mus Musculus (mouse) 
2026-01-31 | PXD063017 | JPOST Repository
ProteomicsLFQ of anti-Myc IP-MS of AIFM1(TCS) or AIFM1(TCS/TEV)-expressing cells
The mitochondrial fractions from the AIFM1(TCS)- and AIFM1(TCS/TEV)-expressing cells were lysed in 1 mL of lysis buffer containing 50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 10% (w/v) glycerol, 1 mM EDTA, 0.5% (w/v) digitonin, and protease inhibitor cocktail. After centrifugation, the clarified supernata...
ORGANISM(S): Homo Sapiens (human) 
2026-01-31 | PXD063094 | JPOST Repository
ProteomicsLFQ of anti-Myc IP-MS of OMA1-Myc-expressing MEFs
OMA1 KO MEFs (10 cm dish) and its rescued cells stably expressing WT or EQ mutant OMA1-Myc were incubated with 0.2% (w/v) formaldehyde for 15 min at 37° C, followed by quenching with 100 mM glycine-NaOH (pH 7.5) for 10 min at room temperature. After once wash with 1× PBS (pH 7.4), the cells were scr...
ORGANISM(S): Mus Musculus (mouse) 
2026-01-31 | PXD063014 | JPOST Repository
ProteomicsIdentification of novel biomarkers for aortic aneurysm
To discover novel biomarkers for aortic aneurysm, serum samples of patients with thoracic aortic aneurysm were fractionated, tryptic digested, and subjected to proteome analysis.
ORGANISM(S): Homo Sapiens (human) 
2023-11-15 | PXD033774 | JPOST Repository
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