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Microarray analysis was performed using germ cells and gonadal somatic cells isolated by Fluorescence Activated Cell Sorter (FACS) from XX and XY embryos at st.33 and st.35. Medaka germ cells (olvas-EGFP) and somatic cells (sox9b-DsRed) were isolated by FACS. The microarray experiments were done us...
ORGANISM(S): Oryzias latipes 
Recent advancements in mass spectrometry-based proteomics have made it possible to conduct comprehensive protein analysis. In particular, the emergence of the data-independent acquisition (DIA) method powered by machine learning has significantly improved protein identification effi-ciency. However,...
ORGANISM(S): Homo Sapiens (human) 
Excisable DD construct was fused with TurboID and transduced into NIH3T3 cells. The cells were exposed to Shield-1 (1 μM) for 24 h, followed by co-treatment of Shield-1 (1 μM) and biotin (50 µM) for 24 h (biological replicates; n = 3). The cell lysates in guanidine-TCEP buffer were dissolved by heat...
ORGANISM(S): Mus Musculus (mouse) 
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