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MetabolomicsNitrate-Sialin2 Axis Couples ER-Mitochondrial Calcium Signaling with Fatty Acid Metabolism to Drive White Adipose Browning
Nutrient cues shape adipose homeostasis; however, the mechanism by which inorganic signals engage organelle networks to drive white fat browning remains unclear. Here, we identify a nitrate-Sialin2 pathway that converts dietary nitrate into a spatially confined thermogenic program. Sialin2 localizes...
2026-05-17 | MTBLS14192 | MetaboLights
TranscriptomicsTranscriptional characterization of maize roots in response to urea and nitrate
Although urea is the most used nitrogen fertilizer worldwide, little is known on the capacity of crop plants to use urea per se as a nitrogen source for development and growth. To date, the molecular and physiological bases of its transport have been investigated only in a limited number of species....
ORGANISM(S): Zea mays 
2015-03-02 | E-GEOD-53102 | biostudies-arrayexpress
ProteomicsThe signal transduction protein PII controls ammonium, nitrate and urea uptake in cyanobacteria
PII signal transduction proteins are widely spread among all domains of life where they regulate a multitude of carbon and nitrogen metabolism related processes. Non-diazotrophic cyanobacteria can utilize a high variety of organic and inorganic nitrogen sources. In recent years, several physiologica...
ORGANISM(S): Synechocystis sp. PCC 6803 
2019-07-10 | PXD013411 | Pride
Urea uptake Nitrogen regulation Abc transporters Nitrate uptake Ammonium uptake Glnb Cyanobacteria Pii signaling protein 
TranscriptomicsDiatom transcriptomics under nitrate limitation
Here, we examined the ramifications of between-species diversity by documenting the transcriptional response of three marine diatoms - Thalassiosira pseudonana, Fragilariopsis cylindrus, and Pseudo-nitzschia multiseries - to the onset of nitrate limitation of growth, a common limiting nutrient in th...
ORGANISM(S): Thalassiosira pseudonana 
2014-03-25 | E-GEOD-56132 | biostudies-arrayexpress
TranscriptomicsComparison of root transcriptomes in Arabidopsis thaliana plants supplied with different forms of inorganic nitrogen
Plants aquire nitrogen from the soil, most commonly in the form of either nitrate or ammonium. Unlike ammonium, nitrate must be reduced (with NADH and ferredoxin as electron donors) prior to assimilation. Thus, nitrate nutrition imposes a substantially greater energetic cost than ammonium nutrition....
ORGANISM(S): Arabidopsis thaliana 
2011-05-28 | E-GEOD-29589 | biostudies-arrayexpress
TranscriptomicsImpact of NRT1.1 point mutants on gene expression under high NH4NO3 supply
NRT1.1 is a nitrate transceptor involved in many nitrate responses including the regulation of gene expression through (i) the Primary Nitrate Response (PNR) and (ii) the regulation the NRT2.1 gene under continuous high NH4NO3 conditions. Phosphorylation of NRT1.1’s T101 residue is involved in the m...
ORGANISM(S): Arabidopsis thaliana 
2015-01-15 | E-GEOD-49437 | biostudies-arrayexpress
TranscriptomicsCHL1 functions as a nitrate sensor and also is one of major nitrate transporters responsible for nitrate responses and uptake.
CHL1 functions as a nitrate sensor and also is one of major nitrate transporters responsible for nitrate responses and uptake. ANI is a protein phosphatase particiates in temporal nitrate response, its influence was analyzed in a global transcriptome study using Affymetrix ATH1 array. Two biological...
ORGANISM(S): Arabidopsis thaliana 
2014-10-26 | E-GEOD-33242 | biostudies-arrayexpress
ProteomicsPhosphoproteome profiling under low nitrate and high nitrate in Arabisopsis wild type roots
In this project, we treated Arabidopsis wild type (Col-0) with low nitrate (0.2 mM KNO3) and high nitrate (5 mM KNO3) after nitrate starvation. Next we harvested root, and then we performed memebrane fraction and cytosolic fraction isolation for trypsin digestion and phosphopeptide enrichment.
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2024-05-21 | PXD014146 | Pride
Phosphoproteomics Nitrate signaling 
TranscriptomicsTranscription profiling of Arabidopsis 10 day old wild type and chl1-5 plants exposed to 25 mM nitrate for 0h or 0.5h
CHL1 is one of major nitrate transporters responsible for nitrate uptake and some studies suggest that it may be also play a role in regulating the nitrate response, its influence was analyzed in a global transcriptome study using Affymetrix ATH1 array. Experiment Overall Design: Three biological re...
ORGANISM(S): Arabidopsis thaliana 
2008-06-16 | E-GEOD-9148 | biostudies-arrayexpress
ProteomicsNitrate Starvation time course membrane protein phosphoproteomics
Nitrate nutrtition was withdrawn from Arabiodpsis plants to study early responses upon nitrate depletion. Plants were grown under full nutrition (3mM nitrate) for three weeks and then transferred to nutrient solution without nitrate for 15 minutes and 3 hours.
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2024-05-21 | PXD015390 | Pride
Arabidopsis Nitrate starvation Membrane proteins Phosphorylation 
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