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ProteomicsIsolation and Characterization of small EVs from CHO-K1 cell cultures
Chinese hamster ovary (CHO) cells are widely used host cells for recombinant protein production and currently the most commonly utilized mammalian organism in large scale biopharmaceutical production. Since the discovery of exosomes as a new type of small extracellular vesicles (EVs) by Johnstone et...
ORGANISM(S): Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) 
2021-09-09 | PXD018446 | Pride
Proteomics Cho Cell culture Extracellular vesicles Exosomes 
ProteomicsTime-resolved proteomic profiling for CHO-K1 fed-batch cultivations
The majority of recombinant protein therapeutics are produced with Chinese Hamster Ovary (CHO) cells. Productivity depends on the initial cell line engineering in terms of integration site or choice of an appropriate promotor for the recombinant gene expression, as well as media and process paramete...
ORGANISM(S): Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) 
2021-01-25 | PXD018439 | Pride
Fedbatch cultivation Nlc-ms/ms Cho Proteomics Dda 
ProteomicsTECAN Resolvex A200 Positive Pressure Workstation for Semi Automated Peptide Purification of Complex Proteomic Samples
High-throughput has become a substantial need in many omics investigations. In proteomics the sample preparation workflow consists of multiple steps, whereby with each additional manual step more bias is added to the sample. Especially for label-free quantification experiments this drastically reduc...
ORGANISM(S): Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) 
2021-01-25 | PXD018444 | Pride
Peptide purification Ppspe Cell culture Fedbatch cultivation High-throughput proteomics Sample preparation 
ProteomicsHyperosmolality in CHO Culture: Effects on Proteome
Chinese hamster ovary cells (CHO) were exposed to highly concentrated feed solution during a fed-batch cultivation which causes an unphysiological osmolality increase (>300 mOsm/kg) affecting cell physiology, morphology and proteome. To get a deeper insight into underlying molecular mechanisms invol...
ORGANISM(S): Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) 
2022-05-20 | PXD027443 | Pride
Nlc-ms lfq proteomics Fed-batch Q exactive plus Cho Hyperosmolality Cell size 
UnknownCancer-associated hypersialylated MUC1 drives the differentiation of human monocytes into macrophages with a pathogenic phenotype.
Not available
S-EPMC7642421 | biostudies-literature
UnknownIn-person vs. web-based administration of a problem-solving skills intervention for parents of children with cancer: Report of a randomized noninferiority trial.
Not available
S-EPMC7327899 | biostudies-literature
UnknownCrystal structure and mechanistic basis of a functional homolog of the antigen transporter TAP.
Not available
S-EPMC5278451 | biostudies-literature
UnknownAlu insertion polymorphisms shared by Papio baboons and Theropithecus gelada reveal an intertwined common ancestry.
Not available
S-EPMC6880559 | biostudies-literature
UnknownCompromised transcription-mRNA export factor THOC2 causes R-loop accumulation, DNA damage and adverse neurodevelopment.
Not available
S-EPMC10853216 | biostudies-literature
UnknownSARS-CoV-2 recombinant spike ferritin nanoparticle vaccine adjuvanted with Army Liposome Formulation containing monophosphoryl lipid A and QS-21: a phase 1, randomised, double-blind, placebo-controlled, first-in-human clinical trial.
Not available
S-EPMC11192176 | biostudies-literature
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