Sort   by:  
 Page size 
MetabolomicsTFAM loss induces nuclear actin assembly upon mDia2 malonylation to promote liver cancer metastasis
The mechanisms underlying cancer metastasis remain poorly understood. Here, we report that TFAM deficiency rapidly and stably induced spontaneous lung metastasis in mice with liver cancer. Interestingly, unexpected polymerization of nuclear actin was observed in TFAM-knockdown HCC cells when cytoske...
2022-06-23 | MTBLS4307 | MetaboLights
TranscriptomicsDeep Sequencing of Human Nuclear and Cytoplasmic Small RNAs Reveals an Unexpectedly Complex Subcellular Distribution of miRNAs and tRNA 3' Trailers
Background: MicroRNAs (miRNAs) are 22-nt small non-coding regulatory RNAs that have generally been considered to regulate gene expression at the post-transcriptional level in the cytoplasm. However, recent studies have reported that some miRNAs localize to and function in the nucleus. Methodology/Pr...
ORGANISM(S): Homo sapiens 
2010-07-14 | E-GEOD-22918 | biostudies-arrayexpress
ProteomicsProteomics analysis of nuclear envelope, cytoplasmic membrane and nuclear contents fractions isolated from mesenchymal stem cells, adipocytes and myocytes
We used shotgun proteomics to analyze three subcellular fractions isolated from mesenchymal stem cells and from differentiated adipocytes and myocytes: nuclear envelopes (NEs), cytoplasmic membranes and nuclear contents. The proteomics data were analyzed by a NSAF-based scoring system to calculate...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2018-11-27 | MSV000083166 | MassIVE
TranscriptomicsKnockdown of SR protein family in P19 cells and RNA-seq of cytoplasmic and whole cell polyA+ RNA
Our data suggest that all SR proteins contribute to mRNA export via NXF1. To identify endogenous export targets we depleted all seven SR proteins individually from P19 WT cells prepared cytoplasmic fractions. We sequenced the cytoplasmic fraction and as a control whole celll RNA from the identical s...
ORGANISM(S): Mus musculus 
2016-03-07 | E-GEOD-69733 | biostudies-arrayexpress
TranscriptomicsEfficient cellular fractionation of RNA
The aim of this experiment was to develop new methods to extract pure fractions of nuclear and cytoplasmic RNA. We compared the results of nuclear and cytoplasmic RNA-sequencing to results of the total and polyA+ RNA-sequencing. Cytoplasmic, nuclear, total and polyA+ RNA was extracted from two human...
ORGANISM(S): Homo sapiens 
2013-10-22 | E-MTAB-1898 | biostudies-arrayexpress
TranscriptomicsNuclear-localized tiny RNAs are associated with transcription initiation and splice sites in metazoans
We have recently shown that transcription initiation RNAs (tiRNAs) are derived from sequences downstream of transcription start sites. Here we report the identification of a second class of nuclear-specific ~17-18 nucleotide small RNA whose 3M-bM-^@M-^Y ends map precisely to the splice donor site of...
ORGANISM(S): Homo sapiens 
2010-07-01 | E-GEOD-20664 | biostudies-arrayexpress
GenomicsTranscriptome of cytoplasmic and nuclear small RNAs in innate immune cells
Transcriptome of cytoplasmic and nuclear small RNAs in innate immune cells
PRJNA683603 | ENA
TranscriptomicsNuclear scaffold and matrix attachment of HeLa chromatin
The nuclear scaffold/matrix provides an anchor for higher order genome structure that has both structural and functional implications. Different extraction protocols, i.e., utilizing either 25 mM LIS or 2 M NaCl, isolate somewhat different protein constituents of either the nuclear scaffold or nucl...
ORGANISM(S): Homo sapiens 
2010-05-22 | E-GEOD-13774 | biostudies-arrayexpress
TranscriptomicsNuclear scaffold and matrix attachment of Aortic Adventitial Fibroblast (AoAF) chromatin
The nuclear scaffold/matrix provides an anchor for higher order genome structure that has both structural and functional implications. Different extraction protocols, i.e., utilizing either 25 mM LIS or 2 M NaCl, isolate somewhat different protein constituents of either the nuclear scaffold or nucl...
ORGANISM(S): Homo sapiens 
2009-03-10 | E-GEOD-13792 | biostudies-arrayexpress
GenomicsCytoplasmic and nuclear fractionation from HEK293T cells
Cytoplasmic and nuclear fractionation from HEK293T cells
PRJNA800803 | ENA
Sort   by:  
 Page size