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ProteomicsLEM2 phase separation promotes ESCRT-mediated nuclear envelope reformation
Quantitative crosslinking analysis to probe in vitro conformational dynamics governing ESCRT-mediated nuclear envelope formation. CHMP7 was crosslinked with D12-BS3 while CHMP7 in the presence of LEM2 winged helix domain was crosslinked with H12-BS3. Samples were mixed after crosslinking and analy...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2020-01-24 | MSV000084837 | MassIVE
ProteomicsComparative membrane proteomics reveals diverse cell regulators concentrated at the nuclear envelope
The nuclear envelope (NE) is a subdomain of the ER with a distinctive protein composition that underpins its role in nuclear compartmentalization. To expand an understanding of NE functions, we refined methods to reveal low abundance transmembrane (TM) proteins concentrated at the NE. De facto, th...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2023-01-25 | MSV000091154 | MassIVE
TranscriptomicsTranscription profiling of C2C12 cells cultured either in 20% serum (undifferentiated) or in 2% horse serum (differentiated) to detail the global changes of gene expression in lamins and nuclear envelope assoicated proteins during myogenesis
During muscle differentiation, myogenesis sepcific genes are differentially regulated, including Lamins that function at least in maintenance of nuclear architecture and regulation of gene expression. We used microarrays to detail the global changes of gene expression in lamins and nuclear envelope ...
ORGANISM(S): Mus musculus 
2007-11-22 | E-GEOD-4694 | biostudies-arrayexpress
ProteomicsProteomics analysis of nuclear envelope, cytoplasmic membrane and nuclear contents fractions isolated from mesenchymal stem cells, adipocytes and myocytes
We used shotgun proteomics to analyze three subcellular fractions isolated from mesenchymal stem cells and from differentiated adipocytes and myocytes: nuclear envelopes (NEs), cytoplasmic membranes and nuclear contents. The proteomics data were analyzed by a NSAF-based scoring system to calculate...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2018-11-27 | MSV000083166 | MassIVE
ProteomicsCombining proximity labeling and crosslinking mass spectrometry for proteomic dissection of nuclear envelope interactome
Analysis of LAP1 interactome in HEK293T cells using proximity labeling or proximity labeling combined with crosslinking methods.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-11-20 | MSV000084606 | MassIVE
TranscriptomicsInducible repositioning of mouse gene tethering to the inner nuclear membrane
Nuclear compartmentalization appears to play an important role in regulating metazoan genes. While studies on immunoglobulin (Ig) and other loci have correlated positioning at the nuclear lamina with gene repression, the functional consequences of this compartmentalization remain untested. We devise...
ORGANISM(S): Mus musculus 
2008-06-11 | E-GEOD-10176 | biostudies-arrayexpress
ProteomicsAdaptive partitioning of a gene locus to the nuclear envelope in Saccharomyces cerevisiae is driven by polymer-polymer phase separation
Partitioning of active gene loci to the nuclear envelope is a key mechanism by which organisms can increase the speed of adaptation and metabolic robustness to fluctuating resources in the environment. In the budding yeast Saccharomyces cerevisiae, adaptation and transcriptional memory induced by nu...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2023-03-01 | PXD029913 | Pride
Active gene Nuclear envelope Ino1 Gene partitioning Chromatin decompaction Active chromatin Phase separation 
ProteomicsA multi-omics atlas of the plant nuclear envelope
The nuclear envelope (NE) proteins play a pivotal role in maintaining nuclear integrity and orchestrating nucleocytoplasmic communication. Increasing evidence suggests that NE proteins interact with a wide array of other proteins, implying functions far beyond membrane compartmentalization. However,...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2026-03-06 | PXD071667 | Pride
Arabidopsis Nuclear envelope Nucleus 
GenomicsThe nuclear envelope protein Net39 is essential for muscle nuclear integrity and chromatin organization
The nuclear envelope protein Net39 is essential for muscle nuclear integrity and chromatin organization
PRJNA647764 | ENA
ProteomicsSearching NE-enriched proteomics datasets for splice junction peptides
MudPIT datasets for NaOH-extracted and Salt-and-Detergent (SD)-extracted rat nuclear membranes were obtained from skeletal muscle and liver tissue as described in the following references: Wilkie GS, Korfali N, Swanson SK, Malik P, Srsen V, Batrakou DG, de las Heras J, Zuleger N, Kerr AR, Florens L,...
ORGANISM(S): Rattus Norvegicus (ncbitaxon:10116) 
2018-01-18 | MSV000081951 | MassIVE
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