LEM2 phase separation promotes ESCRT-mediated nuclear envelope reformation

ABSTRACT: Quantitative crosslinking analysis to probe in vitro conformational dynamics governing ESCRT-mediated nuclear envelope formation. CHMP7 was crosslinked with D12-BS3 while CHMP7 in the presence of LEM2 winged helix domain was crosslinked with H12-BS3. Samples were mixed after crosslinking and analyzed according to standard procedures. The raw files included here correspond to four SEC fractions from a single experiment that contain crosslinked peptides.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Adam Frost  

PROVIDER: MSV000084837 | MassIVE | Fri Jan 24 10:53:00 GMT 2020

REPOSITORIES: MassIVE