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A Pseudomonas aeruginosa-derived neutral ceramidase (PaCDase) isolated from a patient with atopic dermatitis was shown to effectively degrade ceramide in the presence of Staphylococcus aureus-derived lipids or neutral detergents. To understand the effect of ceramide metabolites on the functions of d...
ORGANISM(S): Homo sapiens 
The expression levels of metabolic enzymes were estimated by MRM analysis with internal standard recombinant proteins. The analysis is performed in human diploid fibroblasts, TIG-3 cells and their derived cell lines (TS, TSM, and TSR). TIG-3 TS is immortalized cells by expressing hTERT and SV40 larg...
ORGANISM(S): Homo Sapiens (human) 
The expression levels of metabolic enzymes were estimated by MRM analysis with internal standard recombinant proteins. The analysis is performed in human diploid fibroblasts, TIG-3 cells and their derived cell lines (TS, TSM, and TSR). TIG-3 TS is immortalized cells by expressing hTERT and SV40 larg...
ORGANISM(S): Homo Sapiens (human) 
The expression levels of metabolic enzymes were estimated by MRM analysis with internal standard recombinant proteins. The analysis is performed in human diploid fibroblasts, TIG-3 cells and their derived cell lines (TS, TSM, and TSR). TIG-3 TS is immortalized cells by expressing hTERT and SV40 larg...
ORGANISM(S): Homo Sapiens (human) 
MS/MS spectra assigned to peptides with more than six amino acids were selected from our database and converted into MRM transitions. Overlapping ions with a difference in mass/charge ratio (m/z) of 400 were preferentially selected
ORGANISM(S): Homo Sapiens (human) 
The expression levels of metabolic enzymes were estimated by MRM analysis with internal standard recombinant proteins. The analysis is performed in human diploid fibroblasts, TIG-3 cells and their derived cell lines (TS, TSM, and TSR). TIG-3 TS is immortalized cells by expressing hTERT and SV40 larg...
ORGANISM(S): Homo Sapiens (human) 
Absolute quantification of metabolic enzymes (core metabolic set) was carried out by MRM with internal standard recombinant proteins. Sample digests were labeled with mTRAQdelta0 and internal standard peptides were labeled with mTRAQdelta4.
ORGANISM(S): Homo Sapiens (human) 
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