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TranscriptomicsComparative expression analysis between logarithmic and stationary phase of T. thermophilus HB8 on a synthetic medium
We observed the expression profile of the total mRNA of Thermus thermophilus HB8 wild-type strain grown in a synthetic medium at 70°C. Three wild-type T. thermophilus HB8 strains were pre-cultured at 70°C for 16 h in 3 ml of synthetic medium. The cells (2 ml) were inoculated into 500 ml of the same ...
ORGANISM(S): Thermus thermophilus HB8 
2008-08-03 | E-GEOD-11671 | biostudies-arrayexpress
TranscriptomicsDifferential expression analysis of Thermus thermophilus HB8 wild-type strain in response to zinc
We observed the expression profile of the total mRNA in Thermus thermophilus HB8 wild-type strain at 0 and 30 min after the addition of ZnSO4. Three wild-type strain of T. thermophilus HB8 were pre-cultured at 70 degC for 16 hours in 3 mL of TT medium containing 0.8% polypeptone, 0.4% yeast extract,...
ORGANISM(S): Thermus thermophilus HB8 
2010-04-13 | E-GEOD-20900 | biostudies-arrayexpress
TranscriptomicsComparative expression analysis between TTHB212 deletion mutant and wild-type of T. thermophilus HB8
We observed the expression profile of the total mRNA of TTHB212 deletion mutant of Thermus thermophilus HB8 strain grown in a rich (TR) medium at 70°C compared with that of wild type. Keywords: cell type comparison Three wild-type and mutant T. thermophilus HB8 strains were each pre-cultured at 70Â...
ORGANISM(S): Thermus thermophilus HB8 
2010-05-25 | E-GEOD-10371 | biostudies-arrayexpress
TranscriptomicsSuperSeries for the analysis of transcriptional regulation by Thermus themophilus CsoR (TTHA1719)
We observed the copper response of Thermus thermophilus HB8 wild-type and csoR deficient strain at 0 and 30 min after the addition of 1.25 mM CuSO4, and zinc response of T. thermophilus HB8 wild-type strain at 0 and 30 min after the addition of 1.0 mM ZnSO4. Three wild-type and three csoR deficient ...
ORGANISM(S): Thermus thermophilus HB8 
2010-04-12 | E-GEOD-19521 | biostudies-arrayexpress
TranscriptomicsDifferential expression analysis of T. thermophilus HB8 TTHA0995-deficient strain in response to iron
We observed the expression profile of the total mRNA in Thermus thermophilus HB8 TTHA0995 deficient strain at 0 and 30 min after the addition of FeSO4. Three TTHA0995 deficient strain of T. thermophilus HB8 were pre-cultured at 70 degC for 16 hours in 3 mL of TT medium containing 0.8% polypeptone, 0...
ORGANISM(S): Thermus thermophilus HB8 
2010-10-15 | E-GEOD-21470 | biostudies-arrayexpress
TranscriptomicsDifferential expression analysis of T. thermophilus HB8 wild-type strain in response to iron
We observed the expression profile of the total mRNA in Thermus thermophilus HB8 wild-type strain at 0 and 30 min after the addition of FeSO4. Three wild-type strain of T. thermophilus HB8 were pre-cultured at 70 degC for 16 hours in 4 mL of synthetic medium. The cells (2 mL) were inoculated into 20...
ORGANISM(S): Thermus thermophilus HB8 
2010-10-15 | E-GEOD-21199 | biostudies-arrayexpress
TranscriptomicsExpression analysis of Thermus thermophilus HB8 wild-type strain in response to high growth temperature
In order to observe the heat-shock response of extremely thermohilic bacterium T. thermophilus HB8 strain, we analyzed the altered expression profile of the total mRNA in T. thermophilus HB8 wild-type strain after the growth temperature was shifted from 70°C to 80°C for 30 min. Three wild-type str...
ORGANISM(S): Thermus thermophilus HB8 
2010-10-15 | E-GEOD-21288 | biostudies-arrayexpress
TranscriptomicsmRNA expression analysis of T. thermophilus HB8 wild-type strain cultivated in a synthetic medium for 10 and 20 h
We observed the expression profile of the total mRNA in Thermus thermophilus HB8 wild-type strain at 10 and 20 h. Three wild-type strain of T. thermophilus HB8 were pre-cultured at 70 degC for 16 hours in 3 mL of TT medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and...
ORGANISM(S): Thermus thermophilus HB8 
2010-10-15 | E-GEOD-21183 | biostudies-arrayexpress
TranscriptomicsTranscriptional profiling of the T. thermophilus HB8 response to ethanol
We observed the expression profile of the total mRNA in Thermus thermophilus HB8 wild-type strain at 30 min after the addition of 5% ethanol. Three wild-type strain of T. thermophilus HB8 were pre-cultured at 70 degC for 16 hours in 3 mL of TT medium containing 0.8% polypeptone, 0.4% yeast extract, ...
ORGANISM(S): Thermus thermophilus HB8 
2010-10-15 | E-GEOD-21435 | biostudies-arrayexpress
TranscriptomicsDifferential expression analysis of Thermus thermophilus HB8 wild-type strain in response to NaCl
In order to investigate response of T. thermophilus HB8 wild-type strain for high salt stress, we observed the expression profile of the total mRNA in the strain at 30 min after the addition of NaCl to a final concentration of 0.5% and/or 1.5%, and compared them with that of non-treated cells. Three...
ORGANISM(S): Thermus thermophilus HB8 
2010-10-15 | E-GEOD-21289 | biostudies-arrayexpress
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