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TranscriptomicsGene expression analysis of diabetic kidney cortices obtained from ICAM-1 deficient mice and wild type mice.
In the present study, we aimed to determine the genes involved in inflammatory process of diabetic nephropathy. ICAM-1+/+ and ICAM-1-/- mice aged 8 weeks were divided into four groups: 1) nondiabetic ICAM-1+/+ mice (ND-WT), 2) nondiabetic ICAM-1-/- mice (ND-KO), 3) streptozotocin (STZ)-induced diab...
ORGANISM(S): Mus musculus 
2014-10-08 | E-GEOD-21390 | biostudies-arrayexpress
ProteomicsSynGAP variant from Japanese ALS cohort alters the recruitment of RNA binding proteins
FUS is one of the pathogenic RNA-binding proteins for amyotrophic lateral sclerosis (ALS). We previously reported that FUS stabilized SynGAP mRNA at its 3’UTR and maintained spine maturation and cognitive function in mice. To elucidate whether this mechanism could be pathogenic for ALS, we identifie...
ORGANISM(S): Homo sapiens (Human) 
2023-03-11 | PXD026586 | Pride
Ipsc Motor neuron Als Fus Syngap 
TranscriptomicsSeparation of mouse ES cell-derived mesodermal cells based on the expression of Flk1, PDGFRa and CAR.
We found that mouse ES cell-derived Flk1+ cells could be subdivided into three population by the expression of PDGFRa and CAR (Flk1+PDGFRa-CAR-, Flk1+PDGFRa-CAR+, and Flk1+PDGFRa+CAR+). Therefore, global gene expression analysis was perfomed by microarray to characterize these mesodermal subsets. ...
ORGANISM(S): Mus musculus 
2015-05-22 | E-GEOD-58170 | biostudies-arrayexpress
UnknownA highly photostable and bright green fluorescent protein.
Not available
S-EPMC9287174 | biostudies-literature
ProteomicsLFQ of mNeonGreen-Trap IP-MS from cells expressing HKDC1-mNeonGreen
Cells stably expressing full-length HKDC1-mNeonGreen or △N20 HKDC1-mNeonGreen proteins were grown in a 10-cm dish and were treated with or without antimycin/oligomycin for 3h, and then cross-linked with 0.1% formaldehyde for 10min at room temperature. After cross-linking was quenched with 100mM glyc...
ORGANISM(S): Homo Sapiens (human) 
2024-01-21 | PXD040970 | JPOST Repository
TranscriptomicsResponse of IRF7-deficient peripheral blood mononuclear cells to pH1N1 influenza virus infection
IRF7 plays a critical role in the production and amplification of the antiviral type I and III interferon response. Autosomal recessive IRF7-deficiency resulted in life-threatening influenza disease in a 3-year-old child. We studied the impact of IRF7-deficiency in non-hematopoietic tissues (fibrobl...
ORGANISM(S): Homo sapiens 
2015-04-07 | E-GEOD-66486 | biostudies-arrayexpress
ProteomicsDemonstration of a complementary approach for genetic diagnosis of inborn errors of immunity using proteogenomic analysis
Genetic diagnosis plays a central role in the clinical management of patients with inborn errors of immunity (IEI). We proposed a novel method for diagnosing IEI using PBMC proteomics integrated with targeted RNA sequencing, providing notable insights into the pathogenesis of IEI. Data-independent a...
ORGANISM(S): Homo Sapiens (human) 
2023-11-25 | PXD038352 | JPOST Repository
ProteomicsLFQ of GFP-Trap IP-MS from MEF cells expressing GFP-WIPI2d
Control or GFP-WIPI2d expressing MEFs were treated with or without 0.5uM Apilimod for one hour, and then cross-linked with 0.1% formaldehyde for ten mins at room temperature. After cross-linking was quenched with 100mM glycine for four mins at room temperature, cells were washed with HEPES saline co...
ORGANISM(S): Mus Musculus (mouse) 
2024-06-25 | PXD043701 | JPOST Repository
ProteomicsLFQ of GFP-Trap IP-MS from MEF cells expressing GFP-Rubicon
Control or GFP-Rubicon expressing MEFs were treated with or without 0.5uM Apilimod for one hour, and then cross-linked with 0.1% formaldehyde for ten mins at room temperature. After cross-linking was quenched with 100mM glycine for four mins at room temperature, cells were washed with HEPES saline c...
ORGANISM(S): Mus Musculus (mouse) 
2024-06-25 | PXD043695 | JPOST Repository
UnknownImpact of respiratory bacterial infections on mortality in Japanese patients with COVID-19: a retrospective cohort study.
Not available
S-EPMC10131342 | biostudies-literature
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