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ProteomicsA stress-reduced passaging technique improves the viability of human pluripotent cells
Xeno-free culture systems have expanded the application of human pluripotent stem cells (PSCs). Here we describe an improved method for the subculture of human PSCs. The revised method significantly facilitated the viability of human PSCs by lowering DNA damage and apoptosis, resulting in more effic...
ORGANISM(S): Homo Sapiens (human) 
2022-01-23 | PXD028731 | JPOST Repository
ProteomicsPost-transcriptionally regulated genes are essential for pluripotent stem cell survival
Here, by combining transcriptome and proteome profiling, we identified 228 post-transcriptionally regulated genes with strict regulation at the protein level in PSCs. Among them, 84 genes were found necessary for the survival of several cell types including PSCs, and 20 genes were identified as spec...
ORGANISM(S): Homo Sapiens (human) 
2022-05-10 | PXD028489 | JPOST Repository
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