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Time course of the S. meliloti response to an osmotic upshift elicited by salt (300mM or 400mM) or sucrose (500mM or 700mM)
ORGANISM(S): Sinorhizobium meliloti 
S. meliloti 1021FDC5 was grown at 30ºC in 20 ml of TY broth with shaking to late logarithmic phase (optical density at 600 nm = 1-1.2). After incubation, cells were pelleted, washed twice in MM and resuspended in 2 ml of the latter medium. For time course experiments in liquid, 0.5 ml of the inocula...
ORGANISM(S): Sinorhizobium meliloti 
The two murine double minute (MDM) family members, MDM2 and MDMX are a well-established negative regulator of p53 activity. Under DNA damage conditions, MDM2 and MDMX are phosphorylated near their RING domains (serine 395 at MDM2 and serine 403 at MDMX) and switch to act as p53 positive regulators. ...
ORGANISM(S): Homo sapiens (Human) 
2026-01-05 | PXD060074 | Pride
In the present work, we used a label-free mass spectrometry approach to identify changes in the abundance of Akt-interacting proteins in cardiomyocytes of MetS rats. Using this strategy, we were able to identify specific proteins with evident dissimilarities in their abundance within the Akt complex...
ORGANISM(S): Rattus norvegicus (Rat) 
2020-02-06 | PXD013260 | Pride
Many genes are transcriptionally regulated as a function of the cell cycle; genes needed for mitosis are strongly cell cycle regulated. From yeasts to humans, forkhead transcription factors are involved in mitotic gene expression. However, the mechanisms of regulation are unclear, especially since f...
ORGANISM(S): Schizosaccharomyces pombe 
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