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In bottom-up proteomics, the complexity of proteomes is still a challenge. For deep proteome profiling, multi-dimensional separation strategies and large initial protein amounts are required. Here, we report on an online 2D-LC-MS/MS approach applying strong cation exchange chromatography (SCX) in di...
ORGANISM(S): Homo sapiens (Human) 
2018-10-17 | PXD008562 | Pride
Although current LC-MS technology permits scientists to efficiently screen clinical samples in translational research, e.g. steroids, biogenic amines and even plasma or serum proteomes, in a daily routine, maintaining the balance between throughput and analytical depth is still a limiting factor. A ...
ORGANISM(S): Homo sapiens (Human) 
2021-07-09 | PXD024893 | Pride
Daily biological rhythms are orchestrated by a combination of the intrinsic circadian clock and external food/feeding-related signals which influence metabolism in health and disease. Understanding how and to which extent both circadian and fasting/feeding rhythms contribute to regulating daily phys...
ORGANISM(S): Mus musculus (Mouse) 
2021-01-22 | PXD008944 | Pride
Ultra high pressure liquid chromatography (UHPLC) systems combined with state of the art mass spectrometers have pushed the limit of deep proteome sequencing to new heights making it possible to identify thousands of proteins in a single LC/MS experiment within a few hours. However, the relationship...
ORGANISM(S): Homo sapiens (Human) 
2014-09-30 | PXD000705 | Pride
Ultra high pressure liquid chromatography (UHPLC) systems combined with state of the art mass spectrometers have pushed the limit of deep proteome sequencing to new heights making it possible to identify thousands of proteins in a single LC/MS experiment within a few hours. However, the relationship...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-31 | MSV000080845 | MassIVE
Development of online 2D-LC for native top-down proteomics. Application to human heart tissue.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2025-02-23 | MSV000097200 | MassIVE
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