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TranscriptomicsmRNA Poly(A)-tail Changes Specified by Deadenylation Broadly Reshape Translation in Drosophila Oocytes and Early Embryos
Because maturing oocytes and early embryos lack transcription, posttranscriptional regulatory processes must control their development. To better understand this control, we profiled translational efficiencies and poly(A)-tail lengths throughout Drosophila oocyte maturation and early embryonic deve...
ORGANISM(S): Drosophila melanogaster 
2016-08-08 | E-GEOD-83616 | biostudies-arrayexpress
GenomicsgammaH2Av ChIP-seq in Drosophila stage 10B and 13 follicle cells
We analyzed gamaH2Av ChIP-seq from hand dissected stage 10B and 13 follicle cell nuclei. Egg chambers were dissected from wild-type (OrR) or H2Av[ΔCT] ovaries to assess binding at the Drosophila Follicle Cell Amplicons and across the genome. ChIP-seq of gammaH2Av bound to follicle cell DNA from stag...
ORGANISM(S): Drosophila melanogaster 
2015-06-18 | E-GEOD-66689 | biostudies-arrayexpress
GenomicsChIP-Seq of ORC2 bound to third instar salivary gland DNA in WT and mutant Drosophila, analyzed by Illumina sequencing
We analyzed ORC2 ChIP-Seq from hand dissected salivary glands of wandering third instar larvae from OrR or SuUR Drosophila. Goals were to ascertain the difference in binding profile between salivary glands expressing and not expressing the Supressor of UnderReplication protein. One replicate is incl...
ORGANISM(S): Drosophila melanogaster 
2011-10-18 | E-GEOD-31899 | biostudies-arrayexpress
TranscriptomicsExpression profile of third instar larval fat body and midgut tissues
We use mRNA-seq to transcriptionally profile larval fat body and midgut tissues from Drosophila third instar larvae. These data provide insights into tissue physiology and can be used to identify tissue specific transcripts. Fat bodies from wandering third instar larvae were dissected from ~50 male ...
ORGANISM(S): Drosophila melanogaster 
2010-11-02 | E-GEOD-25025 | biostudies-arrayexpress
TranscriptomicsMurine polyploid trophoblast giant cells (TGCs) from day E9.5 implantation sites (experimental) vs E9.5 diploid embryos (control)
Array-based quantitative genome hybridization (aCGH) was performed to quantify gene copy number across the euchromatic portion of the genome of polyploid TGCs relative to embryonic diploid control cells. DNA was prepared from diploid embryonic cells and purified polyploid TGCs, labeled, and hybridiz...
ORGANISM(S): Mus musculus 
2013-04-30 | E-GEOD-45778 | biostudies-arrayexpress
TranscriptomicsRNA expression profiles of murine polyploid trophoblast giant cells (TGCs) from day 9.5 implantation sites.
We employed RNA-seq to transcriptionally profile a pure population of hand-dissected polyploid TGCs from embryonic day 9.5. These data provide a set of polyploid-specific TGCs transcripts that will aid in the understanding of TGCs differentiation and endoreplication. TGCs were micro-dissected from d...
ORGANISM(S): Mus musculus 
2013-04-30 | E-GEOD-45782 | biostudies-arrayexpress
TranscriptomicsmicroRNA (miRNA) expression profiles of murine polyploid trophoblast giant cells (TGCs) from day 9.5 implantation sites.
We employed miRNA-seq to profile all miRNAs from a pure population of hand-dissected polyploid TGCs from embryonic day 9.5. These data set of polyploid-specific TGCs microRNAs will provide insights into TGCs differentiation and endoreplication. TGCs were micro-dissected from day E9.5 nine implantati...
ORGANISM(S): Mus musculus 
2013-04-30 | E-GEOD-45784 | biostudies-arrayexpress
TranscriptomicsExpression profile of 16C ovarian follicle cells
We use DSN normalized RNA-seq to transcriptionally profile FACS sorted 16C ovarian follicle cells. These data provide insights into the developmental control of gene expression programmed gene amplificaton. Follicle cells were isolated from whole ovaries by trypsinization and filtering and stained w...
ORGANISM(S): Drosophila melanogaster 
2011-05-27 | E-GEOD-29526 | biostudies-arrayexpress
TranscriptomicsExpression profile of third instar larval salivary gland tissue
We use mRNA-seq to transcriptionally profile larval salivary gland tissue from Drosophila third instar larvae. These data provide insights into tissue physiology and can be used to identify tissue specific transcripts. Salivary glands were dissected from 200 wandering third instar larvae and the ass...
ORGANISM(S): Drosophila melanogaster 
2011-10-18 | E-GEOD-33017 | biostudies-arrayexpress
TranscriptomicsCGH Salivary Gland - Y cn bw sp
modENCODE_submission_720 This submission comes from a modENCODE project of David MacAlpine. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: Most terminally differentiated Drosophila tissues are either polyploid or polytene. Unlike normal chromosomes, where the...
ORGANISM(S): Drosophila melanogaster 
2010-05-15 | E-GEOD-17288 | biostudies-arrayexpress
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