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MetabolomicsGlyceraldehyde-3-phosphate dehydrogenase homologs as bifunctional gatekeepers of metabolic segregation in Pseudomonas putida

Metabolically versatile Pseudomonas species can assimilate various glycolytic and gluconeogenic substrates. Simultaneous assimilation is known to involve segregating carbons from each substrate type into different metabolic pathways, but the mechanisms that govern this segregation remain unresolv...

2025-11-03 | MTBLS12468 | MetaboLights
MetabolomicsMetabolomics analysis reveals the participation of efflux pumps and ornithine in the response of Pseudomonas putida DOT-T1E cells to challenge with propranolol
Efflux pumps are critically important membrane components that play a crucial role in strain tolerance in Pseudomonas putida to antibiotics and aromatic hydrocarbons that result in these toxicants being expelled from the bacteria. Here, the effect of propranolol on P. putida was examined by sudden a...
2016-08-23 | MTBLS320 | MetaboLights
MetabolomicsMetabolic analysis of the response of Pseudomonas putida DOT-T1E strains to toluene using Fourier transform infrared spectroscopy and gas chromatography mass spectrometry
To elucidate any observable metabolic alterations during interactions of several strains of Pseudomonas putida (DOT-T1E, and its mutants DOT-T1E-PS28 and DOT-T1E-18) with the aromatic hydrocarbon toluene, metabolomic approaches were employed. Initially, Fourier-transform infrared (FT-IR) spectroscop...
2016-08-23 | MTBLS319 | MetaboLights
TranscriptomicsExpression data from Pseudomonas putida KT2440 (RNA-Seq data)
We used cDNA generated from total mRNA for RNA-Seq analysis (Genome Analyzer II at GATC Biotech AG) to monitore the gene expression of P. putida after a cold shock from 30M-BM-0C to 10M-BM-0C Culture was grown in minimal medium supplemented with succinate at 30M-BM-0C. In mid-exp. phase cells were ...
ORGANISM(S): Pseudomonas putida KT2440 
2011-02-01 | E-GEOD-24175 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of Pseudomonas putida wild type cells and lexA1 and lexA2 mutants after treatment with mitomcin C
The experiment try to elucidate the analysis of the genetic composition of P. putida lexA1 and lexA2 regulons in order to determine the possible relationships and overlaps between both networks. Gene expression patterns in DNA-damaged wild-type cells of P. putida, as well as in its lexA1 and lexA2 m...
ORGANISM(S): Pseudomonas putida 
2007-10-20 | E-MEXP-1187 | biostudies-arrayexpress
TranscriptomicsExpression data from Pseudomonas putida KT2440
In this study we exploited next-generation Illumina sequencing technology (Wang et al., 2009) to refine the current annotation of the KT2440 genome. Transcriptome sequencing data were queried for yet undescribed small RNAs and ORFs and employed to validate predicted operons and gene coordinates. Exp...
ORGANISM(S): Pseudomonas putida KT2440 
2011-03-25 | E-GEOD-24176 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of P. putida KT2440 cultivated at 7 bar and at 7 bar with elevated dissolved oxygen tension
We investigated the changes of gene expression in PHA-producing Pseudomonas putida KT2440 cultivated under elevated pressure (7 bar) and under combined elevated pressure (7 bar) and elevated dissolved oxygen tension by means of DNA microarrays. RNA samples were isolated from cells cultivated in chem...
ORGANISM(S): Pseudomonas putida 
2012-12-03 | E-MEXP-3632 | biostudies-arrayexpress
TranscriptomicsThe IHF regulon of exponentially growing Pseudomonas putida cells
Integration host factor (IHF) sites are largely absent from intergenic regions of ORFs encoding central metabolic functions in Pseudomonas putida mt-2. To gain an insight into this unequal distribution of otherwise abundant IHF-binding sequences, the transcriptome of IHF-plus and IHF-minus cells gro...
ORGANISM(S): Pseudomonas putida 
2012-07-24 | E-GEOD-23499 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of Pseudomonas putida wild type and roxS mutants to define the RoxS/RoxR regulon
Whole-genome transcriptional analysis has been performed to define the P. putida RoxS/RoxR regulon.
Transcriptome profiles of P. putida roxS cells harvested in stationary growth phase were compared with those of the wild-type strain.
ORGANISM(S): Pseudomonas putida 
2009-01-01 | E-MEXP-1684 | biostudies-arrayexpress
TranscriptomicsHigh-resolution analysis of the m-xylene/toluene biodegradation subtranscriptome of Pseudomonas putida mt-2 [RNA-seq]
Pseudomonas putida mt-2 metabolizes m-xylene and other aromatic compounds through the enzymes encoded by the xyl operons of the TOL plasmid pWW0 along with other chromosomally-encoded activities. Tiling arrays of densely overlapping oligonucleotides were designed to cover every gene involved in this...
ORGANISM(S): Pseudomonas putida 
2016-07-07 | E-GEOD-71852 | biostudies-arrayexpress
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