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TranscriptomicsHigh-throughput sequencing of small RNAs in chicken somite embryos
High-throughput sequencing of small RNAs in chicken somite embryos Dissected tissue white leghorn chicken embryos was disaggregated and RNA extracted using the miRVana kit (Ambion). Small RNA fraction between 19 and 24 nt was isolated from 15% denaturing polyacrylamide gel and 15 micro gram was liga...
ORGANISM(S): Gallus gallus 
2009-04-27 | E-GEOD-15513 | biostudies-arrayexpress
TranscriptomicsSequencing random oligonucleotides using standard GA II protocol
Study on sequencing biases introduced by library preparation step, namely by ligases. Comparison between standard Illumina protocol and improved High Definition (HD) protocol. Four replicates for N21 (21 random nucleotides), one replicate for N9 (9 random nucleotides), using either standard Illumina...
ORGANISM(S): synthetic construct 
2012-07-23 | E-GEOD-36540 | biostudies-arrayexpress
TranscriptomicsNovel players in myogenesis identified by target validation for muscle specific microRNAs in C2C12 myoblasts
Search of novel miR-206 target genes in C2C12 cells, based on differential expression of transcripts containing putative miR-206 binding sites. miR-206 candidate target genes were hpothesized to be upregulated in AM206 vs Day2 sample and downregulated in siRNA vs Day 1 sample. total RNA extracted fr...
ORGANISM(S): Mus musculus 
2012-07-22 | E-GEOD-34069 | biostudies-arrayexpress
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