HCT116 cells were transfected with flag-pcDNA3.1 plasmids or flag-MST3 plasmids. Whole-cell extracts were prepared with a lysis buffer, lysed on ice for 10 min, centrifuged at 12,000 rpm for 10 min at 4°C, and the supernatant was collected. The anti-Flag antibody and protein A/G beads were added and...
ORGANISM(S): Homo Sapiens
