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TranscriptomicsBioinformatics approaches for viral metagenomics in plants using short RNAs : model case of study and application to a Cicer arietinum population
Size fractionated small RNA from total RNA extracts of Cicer arietinum leaves and from Nicotiana benthamiana infected by Cymbidium ringspot virus were mixed in a ratio of 1000 to 1 in amount, respectively. The RNA was ligated to adapters, purified again and reverse transcribed. After PCR amplifica...
ORGANISM(S): Cicer arietinum 
2015-01-30 | E-GEOD-63378 | biostudies-arrayexpress
TranscriptomicsHigh-throughput sequencing of small RNAs in Vitis vinifera
Vitis vinifera endogenous small RNAs Size fractionated small RNA from total RNA extracts of Vitis vinifera leaves, inflorescences, tendrils and small berries were ligated to adapters, purified again and reverse transcribed. After PCR amplification the sample was subjected to Solexa/Illumina high thr...
ORGANISM(S): Vitis vinifera 
2010-04-29 | E-GEOD-18405 | biostudies-arrayexpress
TranscriptomicsmicroRNA-target RNA pairs revealed by Parallel analysis of RNA ends in Vitis vinifera
Vitis vinifera RNA degradome Isolated polyadenylated RNA from total RNA extracts of Vitis vinifera leaves, were ligated to 5'-adapter that include san MmeI recognition site. The ligated products were purified again, reverse transcribed and cleaved with MmeI. The 5' fragments were purified from gel ...
ORGANISM(S): Vitis vinifera 
2010-04-29 | E-GEOD-18406 | biostudies-arrayexpress
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