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TranscriptomicsWhole transcriptome analyses of six thoroughbred horses before and after exercising using RNA-Seq
We sequenced the whole mRNA of six thoroughbred horse (Equus caballus) blood and muscle tissues before and after exercising, generating a total of 1.3 billion short reads with 90-bp pair-end sequences from 24 samples. Comparing with current genome annotation, we identified 32,361 unigene clusters sp...
ORGANISM(S): Equus caballus 
2013-05-01 | E-GEOD-37870 | biostudies-arrayexpress
TranscriptomicsChIP-chip of MG1655 with antibody against E. coli RNAP beta subunit under various conditions
We integrated RNAP binding regions (RBRs) and mRNA transcript abundance to determine segments of contiguous transcription originating from promoter regions. To measure RBRs at a genome scale, we employed a ChIP-chip method to E. coli K-12 MG1655 grown in the presence or absence of rifampicin under m...
ORGANISM(S): Escherichia coli str. K-12 substr. MG1655 
2009-11-03 | E-GEOD-15588 | biostudies-arrayexpress
TranscriptomicsChIP-chip of E. coli K-12 MG1655 with antibody against PurR-8myc under various conditions.
We integrated transcription factor binding regions and mRNA transcript abundance to elucidate the PurR regulon experimentally. To measure transcription factor binding at a genome scale, we employed a ChIP-chip method to derivative strains of E. coli K-12 MG1655 harboring PurR-8myc under various con...
ORGANISM(S): Escherichia coli str. K-12 substr. MG1655 
2011-06-14 | E-GEOD-26589 | biostudies-arrayexpress
TranscriptomicsTranscriptome analysis of E.coli MG1655 under multiple growth conditions
Investigation of comprehensive information about the expression level of RNA transcripts across the entire E.coli genome in mulitple growth conditions, including log-phase; stationary phase, heat shock and nitrogen-limiting condition. A fourteen chip study using total RNA recovered from four separa...
ORGANISM(S): Escherichia coli str. K-12 substr. MG1655 
2009-11-03 | E-GEOD-15534 | biostudies-arrayexpress
TranscriptomicsChIP-chip of Geobacter sulfurreducens PCA with antibody against RNAP and RpoD under various conditions
We applied a ChIP-chip approach to elucidate the binding profiles of RNAP and RpoD experimentally under different growth conditions. This technique localizes DNA fragments within DNA-protein complexes enriched by chromatin immunoprecipitation using high-density oligonucleotide tilling arrays. A 21 C...
ORGANISM(S): Geobacter sulfurreducens PCA 
2010-07-01 | E-GEOD-22503 | biostudies-arrayexpress
TranscriptomicsChIP-chip of E. coli K-12 MG1655 with antibody against ArgR-8myc, Lrp-8myc, and TrpR-8myc under various conditions.
We integrated transcription factor binding regions and mRNA transcript abundance to elucidate the ArgR, Lrp, and TrpR regulon experimentally. To measure transcription factor binding at a genome scale, we employed a ChIP-chip method to derivative strains of E. coli K-12 MG1655 harboring ArgR-8myc, L...
ORGANISM(S): Escherichia coli str. K-12 substr. MG1655 
2011-12-01 | E-GEOD-26054 | biostudies-arrayexpress
TranscriptomicsSTAT3-mediated CD133 upregulation promotes hepatocellular carcinoma
We identified a novel mechanism by which IL-6/STAT3 signaling up-regulates CD133 expression and promotes HCC progression. STAT3 activation upregulates the expression of CD133 during liver carcinogenesis. Targeting STAT3-mediated CD133 overexpression may represent a promising therapeutic strategy for...
ORGANISM(S): Homo sapiens 
2014-08-08 | E-GEOD-60194 | biostudies-arrayexpress
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