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Ventricular myocytes were isolated from adult male Sprague-Dawley rats (250-350g) by collagenase digestion. Cells were cultured with recombinant human interleukin 6 for six hours prior to isolation total RNA by RNeasy mini kit (Qiagen). RNA was amplified and labelled using the Illumina TotalPrep R...
ORGANISM(S): Rattus norvegicus 
We analysed data collected as part of the Biomarkers of Acute Serious Illness in Children (BASIC) study. BASIC was a prospective cohort study that enrolled critically ill children admitted to four PICUs in London and Eastern England, UK, during emergency transport by the Children’s Acute Transport S...
ORGANISM(S): Homo sapiens 
Gene Expression Analysis for Modelling Immune Trajectory and Endotype Transition During Pediatric Septic Shock We aimed to characterise the immune trajectory of 19 children with septic shock from first interaction with pediatric critical care teams (TP1) to 48 hours afterwards (TP2). We analysed da...
ORGANISM(S): Homo sapiens 
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