Ventricular myocytes were isolated from adult male Sprague-Dawley rats (250-350g) by collagenase digestion. Cells were cultured with recombinant human interleukin 6 for six hours prior to isolation total RNA by RNeasy mini kit (Qiagen). RNA was amplified and labelled using the Illumina TotalPrep R...
ORGANISM(S): Rattus norvegicus