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MetabolomicsSelective galactose culture condition reveals distinct metabolic signatures in pyruvate dehydrogenase and complex I deficient human skin fibroblasts

INTRODUCTION: A decline in mitochondrial function represents a key factor of a large number of inborn errors of metabolism, which lead to an extremely heterogeneous group of disorders.

OBJECTIVES: To gain insight into the biochemical consequences of mitocho...

2021-10-06 | MTBLS761 | MetaboLights
MetabolomicsSARS-CoV-2 ORF7a Drives Mitochondrial Dysfunction via PDK4 Activation and Complex I Inhibition

SARS-CoV-2 reprograms host metabolism to promote viral replication and evade immune responses. While infection is known to impair mitochondrial function and enhance glycolysis, the role of viral accessory proteins in these alterations remains unclear. Here, we investigate the metabolic impact of ...

2026-07-09 | MTBLS13338 | MetaboLights
OtherSirtuin 4 is a lipoamidase regulating pyruvate dehydrogenase complex activity.
Data from ProteomeXchange, PXD ID: PXD001447. Experiment: SIRT4_MITO_MOCK1, file: SIRT4_MITO_MOCK1_05.mzml. Published as part of Cell. 2014 Dec 18;159(7):1615-25 . From the Abstract: {{i}} ... Here, we establish SIRT4 as a cellular lipoamidase that regulates the pyruvate dehydrogenase complex (PDH)...
ORGANISM(S): Homo_sapiens_viruses, Human 
GPM32320006423 | GPMDB
Resource Reanalysis 
TranscriptomicsEngineering acetyl-CoA supply: Functional expression of a bacterial pyruvate-dehydrogenase complex in the cytosol of Saccharomyces cerevisiae
The energetic (ATP) cost of biochemical pathways critically determines the maximum yield of metabolites of vital or commercial relevance. Cytosolic acetyl-CoA is a key precursor for biosynthesis in eukaryotes and for many industrially relevant product pathways that have been introduced into Saccharo...
ORGANISM(S): Saccharomyces cerevisiae 
2014-11-04 | E-GEOD-59814 | biostudies-arrayexpress
ProteomicsThe sodium leak channel NALCN is regulated by the neuronal SNARE complex
Chemical cross-linking coupled to mass spectrometry was used to study the subunit organization of the complex between human NALCN, FAM155A, SNAP25 and STX1A proteins. Cross-linking data was generated using the reagents disuccinimidyl suberate (DSS), pimelic dihydrazide (PDH), and 4-(4,6-dimethoxy-1,...
ORGANISM(S): Homo sapiens (Human) 
2025-02-20 | PXD053999 | Pride
Chemical cross-linking  dss  pdh  dmtmm  ion channel  sodium leak channel  nalcn  snare complex 
ProteomicsThe human GID complex engages two independent modules for substrate recruitment
Chemical cross-linking coupled to mass spectrometry was used to study two different assemblies of the human E3 ubiquitin ligase complex, GID. The 5mer complex consisted of subunits MAEA, RMND5a, TWA1, WDR26, and RanBP9; the 6mer complex consisted of the subunits of the 5mer complex and the additiona...
ORGANISM(S): Homo sapiens (Human) 
2021-08-25 | PXD024822 | Pride
Chemical cross-linking  dss  pdh  dmtmm  structural proteomics  gid complex 
TranscriptomicsTargeting Unique Metabolic Properties of Breast Tumor Initiating Cells
Bulk cancer cell populations are known to display distinct metabolic properties compared to their normal counterparts. However, relatively little is known about heterogeneity of metabolic properties within tumors. In this study we show that, analogous to some normal stem cells, breast tumor initiati...
ORGANISM(S): Mus musculus 
2014-07-31 | E-GEOD-41286 | biostudies-arrayexpress
ProteomicsA precisely positioned MED12 activation helix stimulates CDK8 kinase activity
Chemical cross-linking coupled to mass spectrometry was used to study binary and ternary complexes involving cyclin-dependent kinase 8 (CDK8), cyclin-C, and subunit 12 of the Mediator complex (MED12). Cross-linking was performed using different cross-linking chemistries: (1) disuccinimidyl suberate ...
ORGANISM(S): Homo sapiens (Human) 
2020-01-16 | PXD015394 | Pride
Chemical cross-linking  dss  pdh  dmtmm  structural proteomics  mediator complex  kinase 
ProteomicsNeuropilin-2 (NRP2) is the receptor for human cytomegalovirus (HCMV) pentameric complex and is responsible for HCMV infection
Chemical cross-linking coupled to mass spectrometry was used to study the complex between the HMCV pentameric complex (gH/gL/UL128/UL130/UL131A) and its receptor, neuropilin-2 (NRP2). Cross-linking was performed using either disuccinimidyl suberate (DSS) or a combination of pimelic acid dihydrazide ...
ORGANISM(S): Homo sapiens (Human) 
2018-07-26 | PXD007185 | Pride
Chemical cross-linking  dss  structural proteomics  human cytomegalovirus  viral entry  receptor proteins 
UnknownThe PI3K/Akt Pathway Regulates Oxygen Metabolism via Pyruvate Dehydrogenase (PDH)-E1α Phosphorylation.
Not available
S-EPMC4529780 | biostudies-literature
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