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Bone marrow mesenchymal stem cells (BMSCs) is a promising cell type for tissue engineering, however, the application of BMSCs are largely hampered for the limited number harvested from bone marrow cells. The methods or strategies that focused on promoting the capacity of BMSCs expansion ex vivo beco...
ORGANISM(S): Homo sapiens (Human) 
2022-11-28 | PXD036093 | Pride
Protein disulfide isomerase, containing thioredoxin (Trx) domains, serves as a vital enzyme responsible for oxidative protein folding (the formation, reduction, and isomerization of disulfide bonds in newly synthesized proteins) in the endoplasmic reticulum (ER). In this study, we identified TgPDI8,...
ORGANISM(S): Toxoplasma Gondii Rh-88 
a pull-down assay using a concatenated tandem array of consensus TF response elements (catTFRE) to trap the TFs expressed in mouse livers following treatment with or without H2O2
ORGANISM(S): Mus Musculus 
In vitro H2O2-sensitivity of CLOCK cysteines was measured with a quantitative chemoproteomic approach termed as QTRP (Quantitative Thiol Reactivity Profiling) as previously reported. In brief, 5 μg of recombinant CLOCK proteins were reduced with 1 mM DTT for 30 min at room temperature. After reactio...
ORGANISM(S): Homo Sapiens Mus Musculus 
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