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TranscriptomicsCD27 signaling in CD8+ T cells in vivo
Wild type or Cd27-/- mice of 8-12 weeks old were immunized intranasally with 500 ug ovalbumin (OVA) protein and 1 ug cholera toxin in 50 ul HBSS. Spleen, draining lymph node (DLN) or lung were isolated at day 3, day 4 or day 8 after immunization. Single cell suspensions of indicated organs were prep...
ORGANISM(S): Mus musculus 
2013-08-18 | E-MTAB-1772 | biostudies-arrayexpress
TranscriptomicsGene expression analysis of B cell differentiation using B cell blasts and developmentally arrested plasmablasts
In vitro generated blasts from B cells deficient in prdm1 were isolated according to whether they were attempting to express Blimp1, reported by a GFP cassette knocked into the prdm1 locus After four days stimulation with CD40L, IL4 and IL5, cells were FACS sorted into GFP+ and GFP- populations, RNA...
ORGANISM(S): Mus musculus 
2013-02-03 | E-GEOD-43213 | biostudies-arrayexpress
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