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ProteomicsProteome-wide multipoint internal calibration curves for mass spectrometry-based quantitative proteomics
Mass spectrometry (MS)-based proteomics is known for its high accuracy in quantifying peptides and proteins using various calibration strategies, including internal and external calibration curves. While external multi-point calibration curves are created from serial dilutions, they often fail to ac...
ORGANISM(S): Homo sapiens (Human) 
2025-02-24 | PXD059628 | Pride
Quantitative Ovarian cancer Multipoint Tmt Calibration curves 
ProteomicsQuantification of Cardiac Troponin I Using ID-LC-MS/MSQuantification of Cardiac Troponin I Using ID-LC-MS/MS
An analytical protocol based on isotope dilution liquid chromatography-tandem mass spectrometry (ID-LC-MS/MS) which includes a peptide-based calibration strategy was developed and validated for accurate determination of cardiac troponin I (cTnI) levels in clinical samples.
ORGANISM(S): Homo sapiens (Human) 
2025-04-08 | PXD055104 | Pride
Picaa Immunoaffinity enrichment Peptide-based calibration Id-ms Ctni Magnetic particles 
ProteomicsRatio accuracy evaluation and correction using tandem mass tags
The multiplexing capabilities of isobaric mass tag based protein quantification, such as Tandem Mass Tags (TMT) or Isobaric Tag for Relative and Absolute Quantitation (iTRAQ) have dramatically increased the scope of Mass Spectrometry (MS) based proteomics studies. Not only does the technology allow ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2018-08-27 | MSV000082855 | MassIVE
UnknownSpheriCal® -ESI: A dendrimer-based nine-point calibration solution ranging from m/z 273 to 1716 for electrospray ionization mass spectrometry peptide analysis.
Not available
S-EPMC7900979 | biostudies-literature
ProteomicsQuantification of cytokines secreted by primary human cells using MRM: evaluation of analytical parameters â?? shotgun secretome data of inflammatory stimulated HUVECs
The determination of secreted proteins may provide highly valuable information about cell functions. While the typical methods for the determination of biologically relevant but low-abundant molecular species still relies on the use of specific antibodies, mass spectrometry-based methods are now gai...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2016-07-22 | MSV000079982 | MassIVE
ProteomicsQuantification of cytokines secreted by primary human cells using MRM: evaluation of analytical parameters â?? shotgun secretome data of untreated HUVECs
The determination of secreted proteins may provide highly valuable information about cell functions. While the typical methods for the determination of biologically relevant but low-abundant molecular species still relies on the use of specific antibodies, mass spectrometry-based methods are now gai...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2016-07-22 | MSV000079983 | MassIVE
ProteomicsMedian Based Absolute Quantification using Fully Unlabelled Generic Internal Standard
By reporting molar abundances of proteins, absolute quantification determines their stoichiometry in complexes, pathways or networks and also relates them to abundances of non-protein biomolecules. Typically, absolute quantification relies either on protein- specific isotopically labelled peptide st...
ORGANISM(S): Homo sapiens (Human) Caenorhabditis elegans 
2021-11-30 | PXD026886 | Pride
Absolute quantification 
ProteomicsS. cerevisiae DDA orbitrap-based benchmark datasets. C4PR_LIV
In this study, label free peptide intensities from a well quantitatively characterised yeast cell lysate were acquired on two orbitrap mass spectrometers (LTQ-Velos and Q Exactive HF). Additionally, samples containing Universal Proteomics Standard and the Proteomics Dynamic range Standard (http://ww...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2017-08-09 | PXD003472 | Pride
Peptide ionization Hydrophobicity Benchmark dataset Coelution Peptide detectability 
UnknownStudies on quantitative phosphopeptide analysis by matrix-assisted laser desorption/ionization mass spectrometry without label, chromatography or calibration curves.
Not available
S-EPMC4225566 | biostudies-literature
ProteomicsA simple peptide quantification approach for MS-based proteomics quality control
Despite its growing popularity and use, bottom-up proteomics remains a complex analytical methodology. Its general workflow consists of three main steps: sample preparation, liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) and computational data analysis. Quality assessment of th...
ORGANISM(S): Homo sapiens (Human) 
2020-07-28 | PXD014524 | Pride
Method optimization Bottom-up proteomics Peptide quantitation Quality control 
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