Proteins were extracted from the perivitelline fluid of the apple snail Pomacea maculata eggs. And then proteins were seperated on SDS-PAGE gel and analyzed by LTQ-Orbitrap Elite coupled to an Easy-nLC.
Upon fertilisation, the egg must have the resources to facilitate successful fertilisation and regulate zygotic growth prior to the activation of the embryonic genome. A key biomarker of egg activation is the synchronised release of of cortical alveoli (CA) from the egg’s cortex into the perivitelli...
