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Looking at the expression of ER alpha in BRCA1 and mutant tumours
ORGANISM(S): Homo sapiens 
Female FVB/N and Tg.Ac (hemizygotes) mice 3-5 weeks of age were purchased from Harlan UK and Taconic UK, respectively. The mice were contained in an isolator and maintained at 19C with 50% relative humidity and a 12-hour light-dark cycle. Following a 1 week acclimatisation period the dorsal skin o...
ORGANISM(S): Mus musculus 
stimulation of whole blood using 5 ng/ml lipopolysaccharide (LPS, gram -ve bacterial endotoxin) or 5 ug/ml CRP-18 (collagen-related peptide; GPO-18). Monocytes respond to stimuli differently. This experiment was designed to elucidate the transcriptional differences in monocytes responding to either ...
ORGANISM(S): Homo sapiens 
Earlier detection is key to reducing cancer deaths. Here we describe a blood test that can detect eight common cancer types through assessment of the levels of circulating proteins and mutations in cell-free DNA. We applied this test, called CancerSEEK, to 1,005 patients with clinically detected can...
Cancer is driven by mutation. Worldwide, tobacco smoking is the major lifestyle exposure that causes cancer, exerting carcinogenicity through 60 chemicals that bind and mutate DNA. Using massively parallel sequencing technology, we sequenced a small cell lung cancer cell line, NCI-H209, to explore t...
Transcriptional profiling of mutants with beneficial in-frame null mutations. The goal of the study is to understand how expression of the mutants and their parental strain differs in conditions where the mutations are beneficial. For each of two conditions (media with either glutamine or alanine a...
ORGANISM(S): Escherichia coli str. K-12 substr. MG1655 
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