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We are investigating the transcriptional response of newborns in response to prenatal arsenic exposure; We used microarrays to detail the global programme of gene expression response due to prenatal arsenic exposure Experiment Overall Design: cord blood was collected at birth from infants whose moth...
ORGANISM(S): Homo sapiens 
Ventricles were removed from 2-day Sprague_Dawley rat litters from which primary cardiac myocytes (>95%) were isolated. Myocytes were either untreated or exposed to either endothelin-1 for 30, 60 or 120 minutes, cycloheximide for 70 or 130 minutes, endothelin-1 for 10 minutes followed by cycloheximi...
ORGANISM(S): Rattus rattus 
In this study, we determined the miRNA expression profile of bovine alveolar macrophages, using next-generation sequencing strategy. On an Illumina HiSeq 2000 machine, we sequenced 8 miRNA libraries, prepared from small RNA fractions of alveolar macrophages isolated from 8 different healthy animals ...
ORGANISM(S): Bos taurus 
To identify novel LXR target genes, we conducted transcriptional profiling studies using RAW264.7 cells ectopically expressing LXRalpha Total RNA was isolated from RAW264.7 macrophages ectopically expressing LXRalpha as described in Venkateswaran et al. (2000); PNAS 97, 12097-12102. Cells were cult...
ORGANISM(S): Mus musculus 
RNA polymerase (Pol) III transcribes many noncoding RNAs (for example, transfer RNAs) important for translational capacity and other functions. We localized Pol III, alternative TFIIIB complexes (BRF1 or BRF2) and TFIIIC in HeLa cells to determine the Pol III transcriptome, define gene classes and r...
ORGANISM(S): Homo sapiens 
The PHACTR1 gene to a range of vascular and neurological diseases, underscoring its pivotal role in biology. As part of a larger multi-omics study, we performed bulk RNA-seq on stable HT1080 cell lines with overexpression or knockdown of PHACTR1, comparing them to scrambled control cells, to underst...
ORGANISM(S): Homo sapiens 
RNA polymerase III transcribes many noncoding RNAs (e.g. tRNAs) important for translational capacity and other functions. Here, we localized RNA polymerase III, alternative TFIIIB complexes (BRF1/2) and TFIIIC in HeLa cells, determining the Pol III transcriptome, defining gene classes, and reveali...
ORGANISM(S): Homo sapiens 
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