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ProteomicsEngineering Corynebacterium glutamicum to produce the biogasoline isopentenol from plant biomass hydrolysates
Background. Many microbial hosts used for the rapid discovery and development of metabolic pathways can have sensitivities to final products and process reagents. Isopentenol, a biogasoline candidate, has an established heterologous gene pathway but is toxic to many microbial hosts. Reagents used in...
ORGANISM(S): Corynebacterium Glutamicum Atcc 13032 
PXD012293 | panorama
ProteomicsExploring Non-Canonical Terpene Space With a Heterologous Lepidopteran Mevalonate Pathway
Lepidoptera (butterflies and moths) make the six-carbon compounds homoisopentenyl pyrophosphate (HIPP) and homodimethylallyl pyrophosphate (HDMAPP) that are incorporated into sixteen, seventeen and eighteen carbon farnesyl pyrophosphate (FPP) analogues. In this work we heterologously expressed the l...
ORGANISM(S): Escherichia Coli 
2019-10-29 | PXD012697 | panorama
ProteomicsBiosynthesis and secretion of the microbial sulfated peptide RaxX and binding to the rice XA21 immune receptor
The rice immune receptor XA21 is activated by the sulfated microbial peptide RaxX (required for activation of XA21-mediated immunity X) produced by Xanthomonas oryzae pv. oryzae (Xoo). Mutational studies and targeted proteomics revealed that RaxX is processed and secreted by the protease/transporter...
ORGANISM(S): Xanthomonas Oryzae Pv. Oryzae 
PXD013389 | panorama
ProteomicsMassively parallel fitness profiling reveals multiple novel enzymes in Pseudomonas putida lysine metabolism
The lysine metabolism of Pseudomonas putida can produce multiple important commodity chemicals and is implicated in rhizosphere colonization. However, despite intensive study, the biochemical and genetic links between lysine metabolism and central metabolism remains unresolved in P. putida. Here, we...
ORGANISM(S): Pseudomonas Putida Kt2440 
PXD011350 | panorama
ProteomicsA polyketide-based biosynthetic platform for diols, amino alcohols and hydroxyacids
Modular polyketide synthases (PKSs) have been proposed as promising megasynthases for retrobiosynthesis because of their fitness for rational carbon skeleton design. However, over the last three decades, all engineered PKSs produce carboxylic acids via terminal thioesterase (TE), which significantly...
ORGANISM(S): Streptomyces 
PXD047663 | panorama
ProteomicsGenome-scale metabolic rewiring to improve titers, rates and yields of the non-native non-ribosomal peptide product indigoidine at scale
High titer, rates, yields (TRY) and scalability are challenging metrics to achieve due to trade-offs between carbon use for growth and production. To achieve these metrics, we took the minimal cut set (MCS) approach that predicts metabolic reactions for elimination to couple metabolite production st...
ORGANISM(S): Pseudomonas Putida Kt2440 
2020-10-24 | PXD021570 | panorama
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