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TranscriptomicsIdentification of miR-21 targets in Jurkat cells by AGO2 immunoprecipitation
In order to identify miR-21 targets by a biochemical high-throughput method, we immunopurified RISC Complex and associated mRNAs in both control and miR-21 overexpressing Jurkat cells. Following transduction of miR-21 encoding construct (or a cognate control vector) in Jurkat cells, we used microarr...
ORGANISM(S): Homo sapiens 
2014-10-15 | E-GEOD-37212 | biostudies-arrayexpress
TranscriptomicsMiR-21 function in T-lymphocytes
T-lymphocyte activation is efficiently mimicked in vitro by treatment with anti CD3 / anti CD28 antibodies. We report miR-21 induction upon CD3/CD28 stimulation of primary T-lymphocytes. In order to assess the function of miR-21 in T-lymphocytes we interfered with miR-21 function by lentiviral trans...
ORGANISM(S): Homo sapiens 
2014-10-15 | E-GEOD-37213 | biostudies-arrayexpress
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