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ProteomicsTarget deconvolution in Plasmodium falciparum using pulldowns
Parasite lysates were centrifuged (20,000 g, 4°C, 15 min). Beads (blank and with linker attached) were washed 3× with water then twice with lysis buffer. The lysate (~2 mg total protein) was first incubated with 2 mg blank beads for 30 min at 4°C with rotating agitation. The lysate was divided into ...
ORGANISM(S): Plasmodium falciparum (isolate 3D7) 
2022-10-15 | PXD033740 | Pride
Pulldown Plasmodium falciparum Drug target Chemical proteomics 
ProteomicsMudPIT analyses of the proteins co-purified with Ada2b-PB-FLAG-HA FLAG-affinity purified from Drosophila melanogaster S2 cells
Protein Complexes Purification- Stable S2 cell lines expressing Ada2b (isoform B; NP_001027151.1) in the pRmHa3-CHA2FL2 (Ada2b-PBH2F2) were maintained at 1-2 e6 cells/mL in SFX medium (HyQ SFX-Insect). Cells stably transfected with empty vector served as negative controls. Protein complexes containi...
ORGANISM(S): Drosophila Melanogaster (ncbitaxon:7227) 
2018-11-20 | MSV000083153 | MassIVE
UnknownAssociations between social drivers of health and breast cancer stage at diagnosis among U.S. Black women.
Not available
S-EPMC12328792 | biostudies-literature
UnknownRNA Splicing Factor Mutations That Cause Retinitis Pigmentosa Result in Circadian Dysregulation.
Not available
S-EPMC7428848 | biostudies-literature
ProteomicsAnalysis of a cancer-associated mutation in the budding yeast Nuf2 kinetochore protein
Saccharomyces cerevisiae kinetochores were isolated from a NUF2-3V5 background via Dsn1-His-Flag purifications. (AAL020924_02154_SBY23028) and (AAL020924_021524_SBY23066) were grown at 30 C and harvested once cell cultures reached OD600nm = ~3. Cells were resuspended in Buffer H (25mM HEPES pH8.0, 2...
ORGANISM(S): Saccharomyces Cerevisiae W303 (ncbitaxon:580240) 
2025-02-14 | MSV000097121 | MassIVE
UnknownTobacco and marijuana use and their association with serum prostate-specific antigen levels among African American men in Chicago.
Not available
S-EPMC7566952 | biostudies-literature
UnknownA pooled analysis of post-diagnosis lifestyle factors in association with late estrogen-receptor-positive breast cancer prognosis.
Not available
S-EPMC4764465 | biostudies-literature
UnknownPARP1 Inhibition Radiosensitizes Models of Inflammatory Breast Cancer to Ionizing Radiation.
Not available
S-EPMC6825563 | biostudies-literature
TranscriptomicsHouse Dust Mediates Gut Microbiome Lactobacillus johnsonii Enrichment and Immune Defense Against Airway Allergens [Set3]
Exposure to dogs in early infancy has been shown to reduce the risk of childhood allergic disease development and dog-ownership is associated with a distinct house dust microbial exposure. Here we demonstrate, using murine models, that exposure of mice toM-BM- dog-associated house dust protects agai...
ORGANISM(S): Mus musculus 
2013-12-20 | E-GEOD-52908 | biostudies-arrayexpress
TranscriptomicsDysregulation of the colonic mucosally-adherent microbiota associates with HIV disease progression.
HIV is known to severely affect the gastrointestinal immune system, in particular compartments of immunity that regulate gut microbial composition. Furthermore, recent studies in mice have shown that dysregulation of the gut microbiome can contribute to chronic inflammation, which is a hallmark of H...
ORGANISM(S): Homo sapiens 
2013-07-10 | E-GEOD-43746 | biostudies-arrayexpress
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