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1Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA 2Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA 5Curriculum in Genetics and Molecular Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA 8Department of Pharmacology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA (1)
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Center for Anatomy and Cell Biology, Medical University of Vienna, Schwarzspanierstrasse 17, A-1090 Vienna, Austria Tel.: +4314016037718 (1)
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Identification of phosphorylation sites in piggyBac transposase N-terminus

The piggyBac transposon system is widely used for biotechnology and genome engineering and is the founding member of a large superfamily of piggyBac-like elements. We investigated the role in transpositon of the nonconserved N-terminus in the piggyBac transposase, including the impact of predicted c...

ORGANISM(S): Homo sapiens (Human)

2023-03-11 | PXD037132 | Pride

Transposition Piggybac

Clonal expansion analysis of transposon insertions by high-throughput sequencing identifies candidate cancer genes in a PiggyBac mutagenesis screen

Somatic transposon mutagenesis in mice is an efficient strategy to investigate the genetic mechanisms of tumorigenesis. The identification of tumor driving transposon insertions traditionally requires the generation of large tumor cohorts to obtain information about common insertion sites. Tumor dri...

ORGANISM(S): Mus musculus

2013-08-28 | E-GEOD-46210 | biostudies-arrayexpress

Comparative genomic hybridization by array of mouse embryonic stem cells with giant piggyBac transposons stably inserted

Detection of copy-number gain of the ES cells with giant piggyBac transposons stably inserted in the genome using Agilent regional high density comparative genomic array with average probe spacing 130 bp. Extracts were made from equal amounts of human lymphoma cell line DNA mixed with mouse ES sampl...

ORGANISM(S): Homo sapiens

2010-09-01 | E-MEXP-2788 | biostudies-arrayexpress

A hyperactive piggyBac transposase for mammalian applications.

Not available

S-EPMC3029773 | biostudies-literature

Transposase N-terminal phosphorylation and asymmetric transposon ends inhibit piggyBac transposition in mammalian cells

PRJNA833459 | ENA

Highly efficient and low-mosaicism piggyBac transgenesis platform for rapid founder phenotyping [Amplicon-seq]

PRJNA1328622 | ENA

Macaca fascicularis

piggyBac Tg monkey

PRJDB18586 | ENA