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TranscriptomicsTranscriptomic profiling of lung monocytes and macrophages in mice
We developed a simplified flow cytometry strategy in order to discriminate monocytes and macrophages in the lung of C57BL/6 mice. Using this strategy, we identified autofluorescent F4/80+ CD11c+ alveolar macrophages, non-autofluorescent CD64+Ly-6C- interstitial macrophages and Ly-6Chi monocytes resi...
ORGANISM(S): Mus musculus 
2017-03-01 | E-MTAB-5012 | biostudies-arrayexpress
TranscriptomicsTranscriptomic profiling of blood human eosinophils in eosinophilic asthmatic compared to healthy individuals
Eosinophils were FACS sorted from blood of 3 healthy and 3 eosinophilic asthmatic individuals. Total RNA was extracted using Direct-zol™ RNA MiniPrep Kit followed by DNase1 digestion and purification on RNeasy MinElute® Spin Columns. Fragmented and biotin-labeled targets were prepared from 1,5ng ...
ORGANISM(S): Homo sapiens 
2018-12-01 | E-MTAB-4660 | biostudies-arrayexpress
TranscriptomicsMicroarray analysis of gene expression in Rabgef1-knockout and control mouse skin
RABGEF1, a guanine nucleotide exchange factor for Rab5 GTPase, can negatively regulate mast cell activation in vitro. In addition, RabGEF1-deficient mice develop morbidity and severe skin inflammation associated with marked increases in skin mast cell numbers (Tam et al., Nat Immunol, 5(8):844-52, 2...
ORGANISM(S): Mus musculus 
2016-10-04 | E-MTAB-5000 | biostudies-arrayexpress
TranscriptomicsscRNA-seq analysis of lung CD64-expressing mononuclear cells, patrolling and classical monocytes from steady-state C57BL/6J mice
We sought to evaluate in an unbiased way the heterogeneity of lung interstitial macrophages and their relationship with alveolar macrophages, lung Ly-6Chi classical monocytes and Ly-6Clo patrolling monocytes, by single cell RNA-Seq.
ORGANISM(S): Mus musculus 
2019-07-31 | E-MTAB-7678 | biostudies-arrayexpress
TranscriptomicsMicroarray analysis on RNA from lung eosinophil subpopulation from naive or HDM-treated mice using Affymetrix Mouse Gene 2.0 ST
As opposed to their well-characterized contributions to inflammatory processes, tissue eosinophils are now also thought to contribute to immune homeostasis at mucosal sites such as the gut. Yet, whether such steady-state eosinophils exist in the lung is currently unclear. In this project, we identif...
ORGANISM(S): Mus musculus 
2016-06-07 | E-MTAB-4084 | biostudies-arrayexpress
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