Transcriptomic profiling of blood human eosinophils in eosinophilic asthmatic compared to healthy individuals

ABSTRACT: Eosinophils were FACS sorted from blood of 3 healthy and 3 eosinophilic asthmatic individuals. Total RNA was extracted using Direct-zol™ RNA MiniPrep Kit followed by DNase1 digestion and purification on RNeasy MinElute® Spin Columns. Fragmented and biotin-labeled targets were prepared from 1,5ng of total RNA using Affymetrix GeneChip® WT Pico Kit. Fragmented and labeled targets were hybridized to Affymetrix GeneChip® Human Gene 2.0 ST Array following standard protocol.

ORGANISM(S): Homo sapiens

SUBMITTER: Dimitri Pirottin 

PROVIDER: E-MTAB-4660 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress