Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcriptomic profiling of blood human eosinophils in eosinophilic asthmatic compared to healthy individuals

ABSTRACT: Eosinophils were FACS sorted from blood of 3 healthy and 3 eosinophilic asthmatic individuals. Total RNA was extracted using Direct-zolâ¢ RNA MiniPrep Kit followed by DNase1 digestion and purification on RNeasy MinEluteÂ® Spin Columns. Fragmented and biotin-labeled targets were prepared from 1,5ng of total RNA using Affymetrix GeneChipÂ® WT Pico Kit. Fragmented and labeled targets were hybridized to Affymetrix GeneChipÂ® Human Gene 2.0 ST Array following standard protocol.

ORGANISM(S): Homo sapiens

SUBMITTER: Dimitri Pirottin

PROVIDER: E-MTAB-4660 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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