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ProteomicsGTSF1 accelerates target RNA cleavage by PIWI-clade Argonaute proteins
Mass Spectrometry data pertaining to manuscript entitled, "GTSF1 accelerates target RNA cleavage by PIWI-clade Argonaute proteins"
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2022-05-19 | MSV000089490 | MassIVE
TranscriptomicsPIWI-associated piRNA in Drosophila melanogaster ovary
Heterochromatin, representing the silenced state of transcription, largely consists of transposon-enriched and highly repetitive sequences. Implicated in heterochromatin formation and transcriptional silencing in Drosophila are PIWI and repeat-associated small interfering RNAs (rasiRNAs). Despite th...
ORGANISM(S): Drosophila melanogaster 
2007-10-24 | E-GEOD-9138 | biostudies-arrayexpress
TranscriptomicsFLX sequencing of Piwi-associated small RNAs extracted from Drosophila ovarian somatic cells (OSC)
piRNAs function in silencing retrotransposons by associating with the PIWI proteins, AGO3, Aub, and Piwi, in Drosophila germlines. Bioinformatics analyses of piRNAs in Drosophila ovaries suggested that piRNAs are produced by two systems, the primary processing pathway and the amplification loop, fro...
ORGANISM(S): Drosophila melanogaster 
2009-10-01 | E-GEOD-15137 | biostudies-arrayexpress
TranscriptomicsThe Function of Piwi is Independent of Its Slicer Activity
In diverse organisms, the highly-conserved Piwi proteins bind to a complex class of small non-coding RNAs called piRNAs, and are essential for germline stem cell maintenance, transposon silencing, fertility, and offspring viability1. By homology with other Argonautes, Piwi proteins have been propose...
ORGANISM(S): Drosophila melanogaster 
2013-02-04 | E-GEOD-34032 | biostudies-arrayexpress
TranscriptomicsThe MID-PIWI module of Piwi proteins specifies nucleotide- and strand-biases of piRNAs
Piwi-interacting RNAs (piRNAs) guide Piwi Argonautes to suppress transposon activity in animal gonads. Known piRNA populations are extremely complex, with millions of individual sequences present in a single organism. Despite this complexity, specific Piwi proteins incorporate piRNAs with distinct n...
ORGANISM(S): Bombyx mori 
2014-07-01 | E-GEOD-55451 | biostudies-arrayexpress
TranscriptomicsPiwi induces piRNA-guided transcriptional silencing and establishment of a repressive chromatin state
In the Metazoan germline, piwi proteins and associated piwi-interacting RNAs (piRNAs) provide a defense system against the expression of transposable elements. In the cytoplasm, piRNA sequences guide piwi complexes to destroy complementary transposon transcripts by endonucleolytic cleavage. However,...
ORGANISM(S): Drosophila melanogaster 
2013-02-11 | E-GEOD-43829 | biostudies-arrayexpress
TranscriptomicsHigh throughput sequencing of Piwi bound piRNAs from Drosophila ovaries in which key factors for primary piRNA biogenesis in somatic support cells were knocked down using RNAi
In Drosophila, PIWI proteins and bound PIWI interacting RNAs (piRNAs) form the core of a small RNA mediated defense system against selfish genetic elements. Within germline cells piRNAs are processed from piRNA clusters and transposons to be loaded into Piwi/Aubergine/AGO3 and a subset of piRNAs und...
ORGANISM(S): Drosophila melanogaster 
2010-10-03 | E-GEOD-23560 | biostudies-arrayexpress
GenomicsImpact of nuclear Piwi elimination on chromatin state in Drosophila melanogaster ovaries
The piRNA-interacting Piwi protein is involved in transcriptional silencing of transposable elements in ovaries of D. melanogaster. Here we characterized the genome-wide effect of nuclear Piwi elimination on the presence of the heterochromatic H3K9me3 mark and HP1a, as well as on the transcription-a...
ORGANISM(S): Drosophila melanogaster 
2014-05-19 | E-GEOD-56347 | biostudies-arrayexpress
TranscriptomicsMouse MOV10L1 associates with Piwi proteins and is an essential component of the piRNA pathway
Piwi-interacting RNAs (piRNAs) are essential for silencing of transposable elements in the germline but their biogenesis is poorly understood. Here we demonstrate that MOV10L1, a germ cell-specific putative RNA helicase, is associated with Piwi proteins. Genetic disruption of the MOV10L1 RNA helicas...
ORGANISM(S): Mus musculus 
2010-06-12 | E-GEOD-21763 | biostudies-arrayexpress
TranscriptomicsThe Yb Body, a Major Site for Piwi-associated RNA Biogenesis and a Gateway for Piwi Expression and Transport to the Nucleus in Somatic Cells
Despite exciting progress in understanding the Piwi-associ-ated RNA (piRNA) pathway in the germ line, less is known about this pathway in somatic cells. We showed previously that Piwi, a key component of the piRNA pathway in Drosophila, is regulated in somatic cells by Yb, a novel protein containing...
ORGANISM(S): Drosophila melanogaster 
2011-02-10 | E-GEOD-26407 | biostudies-arrayexpress
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