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MetabolomicsAn ancient residue metabolomics-based method to distinguish use of closely related plant species in ancient pipes

Residues from ancient artifacts can help identify which plant species were used for their psychoactive properties, providing important information regarding the deep-time co-evolutionary relationship between plants and humans. However, relying on the presence or absence of one or several biomarke...

2020-07-21 | MTBLS890 | MetaboLights
TranscriptomicsArabidopsis plants exposed to combined heat and drought stress
One of the eminent opportunities afforded by modern genomic technologies is the potential to provide a mechanistic understanding of the processes by which genetic change translates to phenotypic variation and the resultant appearance of distinct physiological traits. Indeed much progress has been m...
ORGANISM(S): Arabidopsis thaliana 
2010-05-26 | E-GEOD-9415 | biostudies-arrayexpress
TranscriptomicsExpression analysis of gene silenced barley to identify the function of a novel LRR-RLK (leucine-rich repeat receptor-like kinase)
VIGS (virus-induced gene silencing) was used to silence a putative LRR-RLK, Affy ID Rbaal9i05_at, in barley. Expression patterns in leaves were compared between untreated control leaves, virus treated leaves, VIGS-Rbaal knockdown leaves and VIGS-Rbaal/PDS (phytoene desaturase) co-silenced leaves. ...
ORGANISM(S): Hordeum vulgare 
2011-12-03 | E-GEOD-34102 | biostudies-arrayexpress
MetabolomicsCascading effects of root microbial symbiosis on the development and metabolome of the insect herbivore Manduca sexta L

Root mutualistic microbes can modulate the production of plant secondary metabolites affecting plant–herbivore interactions. Still, the main mechanisms underlying the impact of root mutualists on herbivore performance remain ambiguous. In particular, little is known about how changes in the plant...

2023-09-12 | MTBLS2700 | MetaboLights
TranscriptomicsTranscription profiling of Arabidopsis Pwer::GFP protoplasts with over-expressed dominant negative mutant isoforms of Aux/IAA transcription factors
Transient genetic modification of plant protoplasts is a straightforward and rapid technique for the analysis of numerous aspects of plant biology. One drawback in the analysis of transformed protoplast suspensions is that they are a heterogeneous mix of cells that have and have not been successfull...
ORGANISM(S): Arabidopsis thaliana 
2009-03-07 | E-GEOD-13783 | biostudies-arrayexpress
TranscriptomicsMidgut - CGH Differential Replication
modENCODE_submission_3278 This submission comes from a modENCODE project of David MacAlpine. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We will precisely identify sequence elements that direct DNA replication by using chromatin immunoprecipitation of known ...
ORGANISM(S): Drosophila melanogaster 
2011-03-25 | E-GEOD-28179 | biostudies-arrayexpress
TranscriptomicsFat Body - CGH Differential Replication
modENCODE_submission_3277 This submission comes from a modENCODE project of David MacAlpine. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We will precisely identify sequence elements that direct DNA replication by using chromatin immunoprecipitation of known ...
ORGANISM(S): Drosophila melanogaster 
2011-03-25 | E-GEOD-28178 | biostudies-arrayexpress
TranscriptomicsBAC-trap studies of Purkinje cells in normal and FMR1 mutant mice
The molecular mechanism(s) leading to Purkinje neuron loss in the neurodegenerative disorder Fragile X-Associated Tremor/Ataxia Syndrome (FXTAS) are limited by the complex morphology of this cell type. Purkinje neurons are notoriously difficult to isolate and maintain in culture presenting considera...
ORGANISM(S): Mus musculus 
2014-05-01 | E-GEOD-57034 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of wheat Lr1- and Lr34-mediated leaf rust resistance in wheat
The wheat gene Lr34 confers partial resistance to all races of Puccinia triticina, the causal agent of wheat leaf rust. However, the biological basis for the exceptional durability of Lr34 is unclear. The Affymetrix wheat genome array was used to identify wheat genes differentially expressed in a ...
ORGANISM(S): Triticum aestivum 
2008-11-13 | E-GEOD-9915 | biostudies-arrayexpress
TranscriptomicsDeveloping seeds of M955 low phytic acid barley 7 days after anthesis
Effect of the low phytic acid mutation on gene expression of developing seeds of M955, a low phytic acid barley genotype with >90% reduction in phytic acid in mature seeds. Expression analysis using the Barley1 GeneChip was performed on total RNA from developing seeds (7DAA) of M955 lpa and wt sib-s...
ORGANISM(S): Hordeum vulgare 
2011-11-22 | E-GEOD-33395 | biostudies-arrayexpress
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