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TranscriptomicsPhospho-site mutants of the RNA Polymerase II C-terminal domain alter subtelomeric gene expression and chromatin modification state in fission yeast
Eukaryotic gene expression requires that RNA Polymerase II (RNAP II) gain access to DNA in the context of chromatin. The C-terminal domain (CTD) of RNAP II recruits chromatin modifying enzymes to promoters, allowing for transcription initiation or repression. Specific CTD phosphorylation marks facil...
ORGANISM(S): Schizosaccharomyces pombe 
2016-08-10 | E-GEOD-83426 | biostudies-arrayexpress
TranscriptomicsFissionNet
Splicing profiling of deletion strains compared to wild-type control 1 replicate for each WT/deletion comparison Splicing sensitive S. pombe microarrays (Agilent-027365) were used to compare the splicing profile of various deletion strains relative to wild-type. The ΔSPAC1952.06c strain displayed r...
ORGANISM(S): Schizosaccharomyces pombe 
2015-12-31 | E-GEOD-74354 | biostudies-arrayexpress
TranscriptomicsCytoplasmic polyadenylation is a major regulator of mRNA fate during oogenesis and egg activation.
The GLD-2 class of poly(A) polymerases regulate the timing of translation of stored transcripts by elongating the poly(A) tails of target mRNAs in the cytoplasm. WISPY is a GLD-2 enzyme that acts in the Drosophila female germline and is required for the completion of the egg-to-embryo transition. Th...
ORGANISM(S): Drosophila melanogaster 
2016-05-18 | E-GEOD-81495 | biostudies-arrayexpress
TranscriptomicsCwc23, an essential J-protein critical for pre-mRNA splicing with a dispensable J-domain
J-proteins are structurally diverse, obligatory co-chaperones of Hsp70s, each with a highly conserved J-domain that plays a critical role in stimulation of Hsp70’s ATPase activity. The essential protein, Cwc23, is one of 13 J-proteins found in the cytosol and/or nucleus of Saccharomyces cerevisiae...
ORGANISM(S): Saccharomyces cerevisiae 
2010-06-21 | E-GEOD-18485 | biostudies-arrayexpress
TranscriptomicsRNAseq and Lariat sequencing of different S. pombe growth conditions
We performed lariat sequencing to profile the diversity of spliced RNA lariats in S. pombe identify annotated and alternate introns. Three different growth conditions were used to grow S. pombe wt and S. pombe Δdbr1. Lariat sequencing of the Δdbr1 strains and RNAseq of the wt and Δdbr1 strains were ...
ORGANISM(S): Schizosaccharomyces pombe 
2015-09-03 | E-GEOD-68345 | biostudies-arrayexpress
TranscriptomicsIdentification of RNAs that are physically and functionally associated with RNase P
Numerous RNAs copurify with RNase P and are affected by temperture sensitive mutations in conserved residues with the essential RNA and protein subunits. Specifically, RNase P physically interacts with ribosomal protein mRNAS and the intron-encoded box C/D snoRNAs. Keywords: RNA copurification, tem...
ORGANISM(S): Saccharomyces cerevisiae 
2008-06-01 | E-GEOD-10514 | biostudies-arrayexpress
TranscriptomicsStructural evidence for consecutive Hel308-like modules in the spliceosomal ATPase Brr2
Brr2 is a DExD/H-box helicase responsible for U4/U6 unwinding, a critical step in spliceosomal activation. Brr2 contains an N-terminal domain and two tandem sets of a helicase-like domain followed by a Sec63 domain with unknown function. We determined the crystal structure of the second Sec63 doma...
ORGANISM(S): Saccharomyces cerevisiae 
2010-05-18 | E-GEOD-16135 | biostudies-arrayexpress
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