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Structural study of the polyA signal recognition by the human CPSF using X-ray crystallography, cross-linking MS, pull-downs and fluorescence polarisation assays.
ORGANISM(S): Homo sapiens (Human) 
2017-12-18 | PXD008122 | Pride
We describe PolyA-Seq, a strand-specific method for high-throughput sequencing of the 3' ends of polyadenylated transcripts. PolyA-Seq is as accurate for digital gene expression as existing RNA sequencing approaches, and superior to microarrays. We used the approach to map polyadenylation (polyA) si...
ORGANISM(S): Homo sapiens 
LMO polyA metaT 2016
LMO polyA metaT 2017
Objectives: The sequencing by the PolyA selection is the most common approach for library preparation. With limited amount or degraded RNA, alternative protocols such as the NuGEN have been developed. However, it is not yet clear how the different library preparations affect the downstream analyses ...
ORGANISM(S): Homo sapiens 
PolyA Sequencing of K562 Cells
The 3' ends of most Drosophila melanogaster genes are poorly annotated or are determined by only a single EST or cDNA clone. To enhance the annotation of poly(A) site use in Drosophila, we performed deep sequencing on RNA isolated from 29 dissected tissues using an approach designed to enrich for p...
ORGANISM(S): Drosophila melanogaster 
ncRNA expression, including snoRNAs, across 11 tissues using polyA-neutral amplification
ORGANISM(S): Homo sapiens 
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