Sort   by:  
 Page size 
ProteomicsAnalysis of the Amphibalanus amphitrite adhesive proteome using pressure cycling technology: Method development and the effect of extraction solvents
Background: Marine biofouling negatively impacts industries reliant upon submerged stationary surfaces. The acorn barnacle Amphibalanus amphitrite is a major biofouler that permanently attaches to a wide array of substrates by producing a proteinaceous adhesive at the surface interface. These interf...
ORGANISM(S): Amphibalanus amphitrite (Striped barnacle) (Balanus amphitrite) 
2019-07-04 | PXD012730 | Pride
Proteomics Amphibalanus amphitrite Bioadhesion Pressure cycling technology Biofouling 
ProteomicsHigh-throughput lysis and proteolytic digestion of tissues using pressure cycling technology for proteomics
we describe a protocol for pressure cycling technology (PCT) assisted tissue sample preparation for high-throughput proteomics
ORGANISM(S): Homo Sapiens Mus Musculus 
2021-12-24 | PXD030645 |
ProteomicsRapid processing of archival tissue samples for proteomic analysis using pressure-cycling technology
Advent of mass spectrometry based proteomics has revolutionized our ability to study proteins from biological specimenin a high-throughput manner. Unlike cell line based studies, biomedical research involving tissue specimen is often challenging due to limited sample availability. In addition, inves...
ORGANISM(S): Homo sapiens (Human) 
2018-01-08 | PXD006255 | Pride
Pressure cycling technology Mass spectrometry Barocycler 
Proteomicsproteotypes of 76 breast cancer cell lines by PCT-SWATH
Here we obtained the proteotypes of 76 breast cancer cell lines using pressure cycling technology (PCT) and SWATH mass spectrometry.
ORGANISM(S): Homo sapiens (Human) 
2021-01-13 | PXD004701 | Pride
Breast cancer  pressure cycling technology  swath-ms 
ProteomicsPCT-assisted mass spectrometric quantification of gingival tissue reveals dynamics of protein synthesis during initiation and progression of inflammatory disease
Understanding the progression of periodontal destruction is at the forefront of periodontal research. Therefore, we aimed to capture the dynamics of gingival tissue proteome during initiation and progression of an experimental periodontitis developed by placing silk ligatures on the second molars of...
ORGANISM(S): Mus musculus (Mouse) 
2020-01-09 | PXD014608 | Pride
Experimental periodontitis Gingival tissue Pressure cycling technology Label-free quantitation Proteome Gingival inflammation Periodontitis Pct 
ProteomicsGlycoPCT: quantitative glycoproteomics based on pressure cycling technology reveals distinctive N-glycosylation in human liver with non-alcoholic fatty liver disease
Protein N-glycosylation plays a crucial role in the human liver, impacting key functions like hepatocyte lipid metabolism hepatocyte apoptosis, and inflammatory response. Despite its significance, the site-specific N-glycosylation patterns and their variations of liver biosy samples between healthy ...
ORGANISM(S): Homo Sapiens 
PXD053626 |
ProteomicsProteome mapping of human gingival tissue in health and disease
Gingival tissue proteome is at the forefront of the periodontal research, yet have been hampered by lack of sufficient tissue-extraction methods. Using an emerging technology for tissue homogenization, pressure cycling technology (PCT), we characterize the gingival tissue proteomic profiles of both ...
ORGANISM(S): Homo sapiens (Human) 
2020-09-25 | PXD018029 | Pride
Pressure cycling technology Gingival tissue proteome Chronic periodontitis 
ProteomicsComparison of FFPE and fresh frozen prostate tissues using PCT SWATH
We compared the proteomes of FFPE and fresh frozen tissues (both tumorous and non-tumorous) from 16 prostate cancer patients using pressure cycling technology and SWATH-MS.
ORGANISM(S): Homo sapiens (Human) 
2019-10-07 | PXD004691 | Pride
Prostate cancer  pct-swath  ffpe 
UnknownHigh-throughput proteomic sample preparation using pressure cycling technology.
Not available
S-EPMC9362583 | biostudies-literature
ProteomicsAccelerated Lysis and Proteolytic Digestion of Biopsy-level Fresh Frozen and FFPE Tissue Samples Using Pressure Cycling Technology
We developed an accelerated PCT method with doubled throughput while maintaining digestion efficiency, the number of proteins/peptides identified, as well as reproducibility of protein quantitation, compared to the previously reported method. The accelerated PCT method does not require new chemical ...
ORGANISM(S): Homo Sapiens 
PXD020353 |
Sort   by:  
 Page size