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ProteomicsHigh-throughput lysis and proteolytic digestion of tissues using pressure cycling technology for proteomics
we describe a protocol for pressure cycling technology (PCT) assisted tissue sample preparation for high-throughput proteomics
ORGANISM(S): Homo Sapiens Mus Musculus 
2021-12-24 | PXD030645 |
TranscriptomicsTranscription profiling of P. putida KT2440 cultivated at 7 bar and at 7 bar with elevated dissolved oxygen tension
We investigated the changes of gene expression in PHA-producing Pseudomonas putida KT2440 cultivated under elevated pressure (7 bar) and under combined elevated pressure (7 bar) and elevated dissolved oxygen tension by means of DNA microarrays. RNA samples were isolated from cells cultivated in chem...
ORGANISM(S): Pseudomonas putida 
2012-12-03 | E-MEXP-3632 | biostudies-arrayexpress
ProteomicsHeat and pressure resistance relates to protein folding and aggregation
The locus of heat resistance (LHR) confers extreme heat resistance in E. coli. This study explored the role of the LHR in pressure resistance of E. coli, as well as its relationship with protein folding and aggregation in vivo. The role of LHR was investigated in E. coli MG1655 expressing a ibpA-yfp...
ORGANISM(S): Escherichia coli 
2020-01-27 | PXD011023 | Pride
Food preservation Escherichia coli Pressure resistance Inclusion bodies 
ProteomicsAccelerated Lysis and Proteolytic Digestion of Biopsy-level Fresh Frozen and FFPE Tissue Samples Using Pressure Cycling Technology
We developed an accelerated PCT method with doubled throughput while maintaining digestion efficiency, the number of proteins/peptides identified, as well as reproducibility of protein quantitation, compared to the previously reported method. The accelerated PCT method does not require new chemical ...
ORGANISM(S): Homo Sapiens 
2020-07-16 | PXD020353 |
ProteomicsPCT-assisted mass spectrometric quantification of gingival tissue reveals dynamics of protein synthesis during initiation and progression of inflammatory disease
Understanding the progression of periodontal destruction is at the forefront of periodontal research. Therefore, we aimed to capture the dynamics of gingival tissue proteome during initiation and progression of an experimental periodontitis developed by placing silk ligatures on the second molars of...
ORGANISM(S): Mus musculus (Mouse) 
2020-01-09 | PXD014608 | Pride
Experimental periodontitis Gingival tissue Pressure cycling technology Label-free quantitation Proteome Gingival inflammation Periodontitis Pct 
UnknownSelective cell lysis pressure on rare and abundant prokaryotic taxa across a shelf-to-slope continuum in the Northern South China Sea.
Not available
S-EPMC10734510 | biostudies-literature
TranscriptomicsTranscription profiling of humoral resistance to the CTL after phenotypical and functional study of the resistant clone IGR-HeuR8
From a tumoral line IGR-Heu; resulting from a biopsy of the primitive tumour of a patient reached of a bronchial cancer not with small cells (NSCLC); we established; by successive co-cultures of this line with the autologous CTL (clone T H161); a resistant line to the cytotoxic lysis induced by H16...
ORGANISM(S): Homo sapiens 
2006-01-23 | E-TABM-71 | biostudies-arrayexpress
UnknownUnderstanding Beta-Lactam-Induced Lysis at the Single-Cell Level.
Not available
S-EPMC8372035 | biostudies-literature
ProteomicsRapid processing of archival tissue samples for proteomic analysis using pressure-cycling technology
Advent of mass spectrometry based proteomics has revolutionized our ability to study proteins from biological specimenin a high-throughput manner. Unlike cell line based studies, biomedical research involving tissue specimen is often challenging due to limited sample availability. In addition, inves...
ORGANISM(S): Homo sapiens (Human) 
2018-01-08 | PXD006255 | Pride
Pressure cycling technology Mass spectrometry Barocycler 
ProteomicsSemi-automated positive pressure FASP in 96-well format (PF96) enables highly reproducible and time-efficient proteomics sample preparation of larger cohorts
As novel technologies allow for a substantial increase in the number of LC-MS run per day, proteomics sample preparation appears as new bottle-neck for throughput. To overcome this limitation, we introduce a novel strategy for positive pressure 96-well filter-aided sample preparation (PF96) on a com...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2021-12-03 | PXD024236 | Pride
96-well format Proteomics Sample preparation Automation 
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