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TranscriptomicsCbf11 Regulates Periodic Expression of Genes Required to Prevent Catastrophic Mitosis in the Fission Yeast Cell Cycle
The expression of a large proportion of the fission yeast genome changes periodically with the cell cycle. Several key transcription factors have been identified that regulate these oscillating and interdependent waves of gene expression. However, for a significant number of cell cycle-regulated gen...
ORGANISM(S): Schizosaccharomyces pombe 
2015-09-18 | E-MTAB-2724 | biostudies-arrayexpress
TranscriptomicsGamma delta T cells isolated from spleen of cold-exposed rats against room-temperature placed control
Wistar male rats were exposed of cold for one day or 14 days. Control group was placed into room temperature. Gamma delta T cells were FACS sorted from spleen.
ORGANISM(S): Rattus norvegicus 
2023-10-30 | E-MTAB-13420 | biostudies-arrayexpress
GenomicsCSL ChIP-seq
The expression of a large proportion of the fission yeast genome changes periodically with the cell cycle. Several key transcription factors have been identified that regulate these oscillating and interdependent waves of gene expression. However, for a significant number of cell cycle-regulated gen...
ORGANISM(S): Schizosaccharomyces pombe 
2015-09-18 | E-MTAB-2725 | biostudies-arrayexpress
TranscriptomicsTranscriptome analysis (Agilent microarrays) of WT, cbf11 and cbf12 Schizosaccharomyces pombe strains treated with camptothecin
The aim was to determine expression levels in untreated cells and cells treated with 20 micromolar camptothecin (CPT) for 2 hours. And the to compare the cbf11 and cbf12 deletion mutants with WT. Cells were grown to exponential phase in YES at 32°C, aliquots of cultures were collected (sample \\"con...
ORGANISM(S): Schizosaccharomyces pombe 
2024-04-05 | E-MTAB-13621 | biostudies-arrayexpress
TranscriptomicsRNA-seq of wild type Mycobacterium smegmatis mc2 155 (exponential and stationary phase) and Ms1 deletion strain (exponential and stationary phase)
Ms1 RNA is ~300 nt sRNA that is highly expressed in stationary phase of growth and binds to the RNA polymerase (RNAP) core. We assume that by binding to RNAP, Ms1 could regulate transcription. Our aim was to reveal the most prominent changes in the transcriptome upon entry into stationary phase that...
ORGANISM(S): Mycolicibacterium smegmatis 
2018-11-12 | E-MTAB-7004 | biostudies-arrayexpress
TranscriptomicsChIP-seq of histone H3 and H3K9me2 in WT and dbl2 mutant fission yeast
The aim was to compare the levels of histone H3 dimethylation at lysine 9 (H3K9me2) in wild-type and dbl2 knock-out Schizosaccharomyces pombe cells. Fission yeast cultures were grown in the complex YES medium to exponential phase and chromatin immunoprecipitation was carried out using anti-H3 and an...
ORGANISM(S): Schizosaccharomyces pombe 
2020-10-02 | E-MTAB-9619 | biostudies-arrayexpress
TranscriptomicsRNA-seq of Bacillus subtilis wild type and RNase J1 null strains
RNase J1 is the first nuclease with 5-3 exonuclease activity in bacteria and plays an important role in the maturation and degradation of mRNA. Absence of RNase J1 in cells has effect on cell morphology and growth rate. We did RNA-seq of Bacillus subtilis wild type and RNase J1 null strains (triplic...
ORGANISM(S): Bacillus subtilis subsp. subtilis str. 168 
2019-12-31 | E-MTAB-5660 | biostudies-arrayexpress
GenomicsChIP-seq of RNase J1 null strain
RNase J1 is the first nuclease with 5’-3’ exonuclease activity in bacteria and plays an important role in the maturation and degradation of mRNA. RNase J1 could also play a role in transcription termination of aberrant complexes. RNase J1 could bind to nascent RNA in such complexes, degrade the nasc...
ORGANISM(S): Bacillus subtilis subsp. subtilis str. 168 
2019-12-31 | E-MTAB-5659 | biostudies-arrayexpress
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