Sort   by:  
 Page size 
MetabolomicsMetabolomics guides rational development of a simplified cell culture medium for drug screening against Trypanosoma brucei
In vitro culture methods underpin many experimental approaches to biology and drug discovery. The modification of established cell culture methods to be more biologically relevant, or to optimise growth, is traditionally a laborious task. Emerging metabolomics technology enables rapid evaluation of ...
2015-05-14 | MTBLS49 | MetaboLights
MetabolomicsLiquid chromatography coupled to high-resolution mass spectrometry metabolomics: A useful tool for investigating tumor secretome based on a three-dimensional co-culture model

Three-dimensional (3D) cell culture technologies, which more closely mimic the complex microenvironment of tissue, are being increasingly evaluated as a tool for the preclinical screening of clinically promising new molecules, and studying of tissue metabolism. Studies of metabolites released int...

2023-12-12 | MTBLS5375 | MetaboLights
MetabolomicsStable Isotope-Assisted Metabolomics for Deciphering Xenobiotic Metabolism in Mammalian Cell Culture
Xenobiotics are ubiquitous in the environment and modified abundantly in the human body by phase I and II metabolism. Liquid chromatography coupled to high resolution mass spectrometry is a powerful tool to investigate these biotransformation products. Here, we present a workflow based on stable iso...
2020-04-14 | MTBLS1358 | MetaboLights
MetabolomicsMetabolomics revealed the influence of breast cancer on lymphatic endothelial cell metabolism, metabolic crosstalk, and lymphangiogenic signaling in co-culture

Breast cancer metastasis occurs via blood and lymphatic vessels. Breast cancer cells 'educate' lymphatic endothelial cells (LECs) to support tumor vascularization and growth. However, despite known metabolic alterations in breast cancer, it remains unclear how lymphatic endothelial cell metabolis...

2024-06-25 | MTBLS859 | MetaboLights
MetabolomicsSelective galactose culture condition reveals distinct metabolic signatures in pyruvate dehydrogenase and complex I deficient human skin fibroblasts

INTRODUCTION: A decline in mitochondrial function represents a key factor of a large number of inborn errors of metabolism, which lead to an extremely heterogeneous group of disorders.

OBJECTIVES: To gain insight into the biochemical consequences of mitocho...

2021-10-06 | MTBLS761 | MetaboLights
MetabolomicsMetabolomic signatures in blastocyst spent culture medium as noninvasive predictors of live birth: a pilot study

Research Question: Can metabolomic profiling of blastocyst spent culture medium (SCM) serve as a non-invasive tool to predict live birth?

Design: This pilot study investigated the association between metabolite profiles in SCM and clinical outcomes following single blastocyst transfer. Seve...

2026-02-13 | MTBLS13898 | MetaboLights
MetabolomicsMarine metabolomics: measurement of metabolites in seawater by gas chromatography mass spectrometry (Cell culture sampling)

One of the major bottlenecks in describing marine ecosystems is characterizing the metabolome of seawater, as salt prevents metabolite analysis. We present SeaMet, a method that can detect hundreds of metabolites in less than one ml of seawater and quantifies them down to nano-molar levels using ...

2019-11-15 | MTBLS843 | MetaboLights
MetabolomicsPrimary Osteocyte Supernatants Metabolomic Profiling of Two Transgenic Mice with Connexin43 Dominant Negative Mutants

Osteocytes could release some small molecules (≤ 1 kDa) through gap junctions and hemichannels to extracellular environment, such as prostaglandin E2 (PGE2), nitric oxide (NO) and adenosine triphosphate (ATP), which play key roles in transferring signals between bone cells and other tissue cells....

2021-04-14 | MTBLS2358 | MetaboLights
MetabolomicsInfluence of Culture Conditions on Metabolite Diversity in Epichloë sinensis Strains

Epichloë sinensis, associated with Festuca sinensis, exhibits rich morphological, genetic and functional diversity across different strains. To comprehensively evaluate the categories and difference in the secondary metabolites of different E. sinensis strains and thereby...

2026-01-04 | MTBLS13598 | MetaboLights
MetabolomicsInvestigating the Impact of Acinetobacter lwoffii-ECT1/E6E7 Co-culture Metabolites on Cervical Cell Proliferation and Apoptosis via Untargeted Metabolomics.

This study employed untargeted metabolomics detection combined with bioinformatics analysis to investigate the metabolites from the co-culture of Acinetobacter lwoffiiand cervical precancerous cells ECT1/E6E7. A. lwoffiiwas co-cultured with ECT1/E6E7 cells at an MOI of 100. The culture supernatan...

2025-12-17 | MTBLS13526 | MetaboLights
Sort   by:  
 Page size