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TranscriptomicsMultiplexed RNA structure characterization with selective 2'-hydroxyl acylation analyzed by primer extension sequencing (SHAPE-Seq)
New regulatory roles continue to emerge for both natural and engineered noncoding RNAs, many of which have specific secondary and tertiary structures essential to their function. Thus there is a growing need to develop technologies that enable rapid characterization of structural features within com...
ORGANISM(S): Bacillus subtilis 
2011-08-26 | E-GEOD-31573 | biostudies-arrayexpress
TranscriptomicsAllele special primer extension microarray for test 12 SNPs contributed to NSCL/P in northeast China.
Nonsyndromic cleft lip with or without palate (NSCL/P) are common birth defects of complex etiology. Multiple interacting loci with possible additional environment factors influence the risk for NSCL/P. 12 single nucleotide polymorphisms (SNPs) in 7 candidate genes were tested using an allele-specif...
ORGANISM(S): Homo sapiens 
2013-04-09 | E-GEOD-45770 | biostudies-arrayexpress
GenomicsOptimizing genome editing strategy by primer-extension-mediated sequencing
Optimizing genome editing strategy by primer-extension-mediated sequencing
PRJNA477827 | ENA
Genomicssynthetic construct
Synthetic construct primer extension products
PRJNA171057 | ENA
UnknownDeep sequencing of non-enzymatic RNA primer extension.
Not available
S-EPMC7337528 | biostudies-literature
UnknownPrimer initiation and extension by T7 DNA primase.
Not available
S-EPMC1462978 | biostudies-literature
UnknownCrystallographic observation of nonenzymatic RNA primer extension.
Not available
S-EPMC5980232 | biostudies-literature
OtherOptimizing genome editing strategy by primer-extension-mediated sequencing
High specificity of e\ngineered nucleases ensures precise genome editing. Couple methods were developed to identify off-target sites of CRISPR/Cas9, but hardly any high-throughput sequencing method can unequivocally determine their targeting efficiencies. Here we describe a comprehensive method, pri...
ORGANISM(S): Homo sapiens 
2019-04-03 | GSE116231 | GEO
OtherMultiplexed RNA structure characterization with selective 2'-hydroxyl acylation analyzed by primer extension sequencing (SHAPE-Seq)
New regulatory roles continue to emerge for both natural and engineered noncoding RNAs, many of which have specific secondary and tertiary structures essential to their function. Thus there is a growing need to develop technologies that enable rapid characterization of structural features within com...
ORGANISM(S): Bacillus subtilis 
2011-08-26 | GSE31573 | GEO
UnknownNon-enzymatic primer extension with strand displacement.
Not available
S-EPMC6872209 | biostudies-literature
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