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TranscriptomicsTranscription profiling of Trypanosoma cruzi transfected with TcPUF6, a pumilio like protein
Functional genomic characterization of mRNAs associated with TcPUF6, a pumilio-like protein from Trypanosoma cruzi
ORGANISM(S): Trypanosoma cruzi 
2008-03-31 | E-MEXP-1355 | biostudies-arrayexpress
TranscriptomicsTcRBP40 pull-down ribonomics
RNA pull-down assay.
For the recombinant protein pull-down assays, 50 M-5g of recombinant His-tag TcRBP40 protein were bound to 100 uL of Ni-NTA resin (Qiagen) overnight at 4M-0C. 100 M-5g of total RNA from epimastigotes were incubated with the bound protein in 500 M-5l EMSA buffer at 4M-0C for 2...
ORGANISM(S): Trypanosoma cruzi 
2012-06-30 | E-MEXP-3057 | biostudies-arrayexpress
TranscriptomicsCBAvsC57macrophagesInfected_LeishmaniaAmazonensis
Employing a transcriptomic approach, the gene expression profiles of C57BL/6 and CBA macrophages, before and after L. amazonensis infection in vitro, were compared. These strains were selected due to their different degrees of susceptibility to this parasite.
ORGANISM(S): Mus musculus 
2012-10-31 | E-MEXP-3448 | biostudies-arrayexpress
TranscriptomicsRNA immunoprecipitation of Trypanosoma cruzi to study messenger ribonucleoproteins (mRNPs) containing TcDhh1.
To study the composition of mRNPs containing TcDhh1, we carried out immunoprecipitation assays with anti-TcDhh1 and epimastigotes lysates. Pre-immune serum was used as control. We also carried out a ribonomic approach to identify the mRNAs present within the TcDhh1 immunoprecipitated complexes. For ...
ORGANISM(S): Trypanosoma cruzi 
2010-10-31 | E-MEXP-2448 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of differential gene expression during Metacyclogenesis in 2 developmental stages in T.cruzei
Evaluation of differential gene expression during the metacyclogenesis process in Trypanosoma cruzi, comparing three stages (epimastigotes, stressed epimastigotes and 24h adhered epimastigotes in differentiation) and using both total and polysomal mRNA.
ORGANISM(S): Trypanosoma cruzi 
2006-05-16 | E-MEXP-92 | biostudies-arrayexpress
TranscriptomicsRNA sequencing of primary isolated unstimulated murine splenic cDC from specific pathogen free (SPF), Germ Free (GF) and Interferon alpha/beta receptor 1 (Ifnar) knock out mice.
The transcriptome of primary isolated unstimulated murine splenic cDC from specific pathogen free (SPF) was compared to cDC from Germ Free (GF) and Interferon alpha/beta receptor 1 (Ifnar) knock out mice. Cells were isolated and sorted by flow cytometry to high purity. Total RNA was isolated using t...
ORGANISM(S): Mus musculus 
2020-03-30 | E-MTAB-8882 | biostudies-arrayexpress
UnknownNew loci associated with kidney function and chronic kidney disease.
Not available
S-EPMC2997674 | biostudies-literature
UnknownIntegration of genome-wide association studies with biological knowledge identifies six novel genes related to kidney function.
Not available
S-EPMC3607468 | biostudies-literature
UnknownMeta-analysis of genome-wide association studies identifies three new risk loci for atopic dermatitis.
Not available
S-EPMC3272375 | biostudies-literature
UnknownGenome-wide association and functional follow-up reveals new loci for kidney function.
Not available
S-EPMC3315455 | biostudies-literature
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