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MetabolomicsProkaryotic viperins produce diverse antiviral molecules
Viperin is an interferon-induced cellular protein conserved in animals. It was shown to inhibit the replication of multiple viruses by producing a ribonucleotide called 3’-deoxy-3’4’-didehydro-CTP (ddhCTP), which acts as a chain terminator for the viral RNA polymerase. Here we show that the eukaryot...
2020-08-24 | MTBLS1750 | MetaboLights
TranscriptomicsAn optimized RNA amplification method for prokaryotic expression profiling analysis
Conventional prokaryotic RNA labeling method usually requires large amounts of starting materials and tends to generate high background signals. Recently, two novel methods based on amplification systems were introduced. Here, we compared three alternative strategies: direct labeling method, ployade...
ORGANISM(S): Escherichia coli 
2009-07-25 | GSE17074 | GEO
TranscriptomicsSensing prokaryotic mRNA signifies microbial viability and promotes immunity
MyD88-independent signal transduction associated with Toll-like receptors (TLRs) 3 and TLR4 is mediated through the adapter protein TRIF (TIR-domain-containing adapter-inducing interferon-beta). It has been proposed that TLR signalling is important for the transcription of crucial inflammasome compo...
ORGANISM(S): Mus musculus 
2011-05-22 | E-GEOD-27960 | biostudies-arrayexpress
TranscriptomicsGene Expression in Prokaryotic Microbial Assemblages Across Salinity Gradients in the Columbia River Coastal Margin
Analysis of microbial gene expression in response to physical and chemical gradients forming in the Columbia River, estuary, plume and coastal ocean was done in the context of the environmental data base. Gene expression was analyzed for 2,234 individual genes that were selected from fully sequenced...
ORGANISM(S): Bacteria uncultured prokaryote Archaea 
2010-09-10 | GSE18303 | GEO
TranscriptomicsAn optimized RNA amplification method for prokaryotic expression profiling analysis
Conventional prokaryotic RNA labeling method usually requires large amounts of starting materials and tends to generate high background signals. Recently, two novel methods based on amplification systems were introduced. Here, we compared three alternative strategies: direct labeling method, ployade...
ORGANISM(S): Escherichia coli 
2010-05-15 | E-GEOD-17074 | biostudies-arrayexpress
TranscriptomicsProkaryotic Single-Cell RNA Sequencing by In Situ Combinatorial Indexing
We present Prokaryotic Expression-profiling by Tagging RNA In Situ and sequencing (PETRI-seq), a high-throughput prokaryotic scRNA-seq pipeline. We demonstrated that PETRI-seq effectively barcoded single bacterial cells in a species-mixing experiment with E. coli (MG1655) and S. aureus (USA300). Wit...
ORGANISM(S): Escherichia coli Staphylococcus aureus 
2020-05-25 | GSE141018 | GEO
TranscriptomicsTranscriptome data of interacting Salmonella Typhimurium and human host cells
The poly(A)+ and poly(A)− fractions of interacting and non-interacting cells were used for distinct library preparation of interacting and non-interacting prokaryotic pathogen and eukaryotic host cells by deepSuperSAGE. Sequencing was performed with the Illumina HiSeq 2000 platform, and one point of...
ORGANISM(S): Salmonella enterica 
2015-05-12 | E-GEOD-61730 | biostudies-arrayexpress
TranscriptomicsPotential Differences in the Metabolic Status of Geobacter sulfurreducens at Different Depths in Anode Biofilms
Microtoming Coupled with Microarray Analysis to Evaluate Potential Differences in the Metabolic Status of Geobacter sulfurreducens at Different Depths in Anode Biofilms Differences in the Metabolic Status of Geobacter sulfurreducens at Different Depths in A Current Producing Biofilm Further insigh...
ORGANISM(S): Geobacter sulfurreducens 
2010-06-21 | E-GEOD-17591 | biostudies-arrayexpress
UnknownA simple, dual direct expression plasmid system in prokaryotic and mammalian cells.
Not available
S-EPMC10165797 | biostudies-literature
UnknownA simple and dual expression plasmid system in prokaryotic (E. coli) and mammalian cells.
Not available
S-EPMC6497378 | biostudies-literature
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