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MetabolomicsRecombinant Production of Biliverdin IXβ and δ Isomers in the T7 Promoter Compatible Escherichia coli Nissle
The ability to obtain purified biliverdin IX (BVIX) isomers other than the commercially available BVIXα is limited due to the low yields obtained by the chemical coupled oxidation of heme. Chemical oxidation requires toxic chemicals, has very poor BVIX yields (<0.05%), and is not conducive to scalab...
2021-12-16 | MTBLS3555 | MetaboLights
TranscriptomicsEnhancer-bound LDB1 Regulates a Corticotrope Promoter Pausing Repression Program [GRO-seq]
Substantial evidence supports the hypothesis that enhancers are critical regulators of cell type determination, orchestrating both positive and negative transcriptional programs; however, the basic mechanisms by which enhancers orchestrate interactions with cognate promoters during activation and re...
ORGANISM(S): Mus musculus 
2015-02-03 | E-GEOD-64515 | biostudies-arrayexpress
TranscriptomicsAnalysis of HSF2 target genes using a global promoter ChIP-chip strategy on mouse wild-type testis
Mapping of in vivo targets for HSF2 by using mammalian tissue material in a global ChIP-chip promoter screen. Chromatin was isolated from three wild-type (WT) mouse testes, crosslinked and sonicated into fragments of 100-500 bp, and the quality of the DNA was controlled before the immunoprecipitatio...
ORGANISM(S): Mus musculus 
2008-08-05 | E-GEOD-9289 | biostudies-arrayexpress
GenomicsThe transcription factor GABP selectively binds and activates the mutant TERT promoter in cancer
Reactivation of telomerase reverse transcriptase (TERT) expression enables cells to overcome replicative senescence and escape apoptosis, fundamental steps in the initiation of human cancer. Multiple cancer types, including up to 83% of glioblastomas (GBM), harbor highly recurrent TERT promoter mut...
EGAS00001001242 | EGA
TranscriptomicsPromoter Capture Hi-C of mouse ESC and TSC
Chromatin organisation of trophoblast stem cells (TSC) were compared with that of embryonic stem cells (ESC). The method enriches Hi-C libraries, to detect promoter interactions at restriction fragment level. We prepared Hi-C libraries from TSC and ESC (serum grown) samples and enriched them with a ...
ORGANISM(S): Mus musculus 
2018-09-05 | E-MTAB-6585 | biostudies-arrayexpress
TranscriptomicsGenome-wide Definition of Promoter and Enhancer Usage During Neural Induction of Human Embryonic Stem Cells [CAGE-seq]
Genome-wide mapping of transcriptional regulatory elements are essential tools for the understanding of the molecular events orchestrating self-renewal, commitment and differentiation of stem cells. We combined high-throughput identification of nascent, Pol-II-transcribed RNAs by Cap Analysis of Gen...
ORGANISM(S): Homo sapiens 
2015-05-21 | E-GEOD-61264 | biostudies-arrayexpress
GenomicsInhibiton of the GABPB1L-containing GABP tetramer is sufficient to reverse replicative immortality in TERT promoter mutant glioblastoma cells.
Over 50 types of cancer acquire TERT promoter mutations. These single point mutations reactivate telomerase, allowing for indefinite maintenance of telomere length and enabling cellular immortalization. The transcription factor binding site created by the point mutations specifically recruit the ETS...
EGAS00001002582 | EGA
GenomicsGenome wide promoter methylation analysis of mouse macrophages and endothelial cells isolated from ischemic hindlimbs using RRBS sequencing
Purpose: The goal of this study was to compare the genome-wide promoter methylation alterations in macrophages and endothelial cells during hindlimb ischemia among normal, hyperlipidemic and type-2 diabetic mice. Methods: Unilateral hindlimb ischemia was induced by ligating femoral artery proximal t...
ORGANISM(S): Mus musculus 
2015-12-21 | E-GEOD-65759 | biostudies-arrayexpress
GenomicsPromoter Capture-HiC in Ring1a KO and Ring1a/b dKO mouse ES cells
Genome organization influences transcriptional regulation by facilitating interactions between gene promoters and distal regulatory elements. To analyse distal promoter contacts mediated by the PRC1 complex we used Capture Hi-C (CHi-C) to enrich for promoter-interactions in a HiC library in Ring1a K...
ORGANISM(S): Mus musculus 
2015-08-01 | E-MTAB-3109 | biostudies-arrayexpress
TranscriptomicsNucleotide composition-linked divergence of vertebrate core promoter architecture
Transcription initiation involves the recruitment of basal transcription factors to the core promoter. A variety of core promoter elements exists, however for most of these motifs the distribution across species is unknown. Here we report on the comparison of human and amphibian promoter sequences. ...
ORGANISM(S): Xenopus (Silurana) tropicalis 
2010-12-30 | E-GEOD-21482 | biostudies-arrayexpress
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