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MetabolomicsThe Bardet-Biedl protein Bbs1 controls photoreceptor outer segment protein and lipid composition

Summary

Primary cilia are key sensory organelles whose dysfunction leads to ciliopathy disorders such as Bardet-Biedl syndrome (BBS). Retinal degeneration is common in ciliopathies, since the outer segments (OSs) of photoreceptors are highly specialized primary cilia. BBS...

2022-02-18 | MTBLS4013 | MetaboLights
Metabolomics3D'omics: Mannan fibre swine trial & Protein digestibility swine trial

Mannan fibre swine trial, BioProject PRJEB86261 at https://www.ebi.ac.uk/ena/browser/view/PRJEB86261

Protein digestibility swine trial, BioProject PRJEB86262 at https://www.ebi.ac.uk/ena/browser/view/PRJEB86262



2025-12-19 | MTBLS13348 | MetaboLights
ProteomicsArabidopsis thaliana peptide SP2 and ethyl acetate extraction clean up.
The success of bottom-up proteomic analysis frequently depends on the efficient removal of contaminants from protein or peptide samples before LC-MS/MS. For a peptide clean-up workflow, the single-pot solid-phase-enhanced peptide sample preparation on carboxylate-modified paramagnetic beads (termed ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2024-01-03 | PXD045031 | Pride
Arabidopsis thaliana Peptide clean-up Ethyl acetate extraction Sp2 Polyethylene glycol Sodium dodecyl sulfate Magnetic beads Lc-ms/ms 
ProteomicsDifferences in Protein Capture by SP3 and SP4 Demonstrate Mechanistic Insights of Proteomics Clean-up Techniques.
The goal of proteomics experiments is to identify proteins to observe changes in cellular processes and diseases. One challenge in proteomics is the removal of contaminants following protein extraction, which can limit protein identifications. Single-pot, solid-phase-enhanced sample preparation (SP3...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2024-02-20 | MSV000094130 | MassIVE
UnknownDifferences in Protein Capture by SP3 and SP4 Demonstrate Mechanistic Insights of Proteomics Clean-up Techniques.
Not available
S-EPMC10980087 | biostudies-literature
UnknownComparative Analysis of Proteoform Clean-Up Methods for In-Depth Top-Down Proteomics.
Not available
S-EPMC12716119 | biostudies-literature
UnknownImmunocapture sample clean-up in determination of low abundant protein biomarkers - a feasibility study of peptide capture by anti-protein antibodies.
Not available
S-EPMC9097496 | biostudies-literature
UnknownAutomated download and clean-up of family-specific databases for kmer-based virus identification.
Not available
S-EPMC8097684 | biostudies-literature
UnknownBenchmarking of Two Peptide Clean-Up Protocols: SP2 and Ethyl Acetate Extraction for Sodium Dodecyl Sulfate or Polyethylene Glycol Removal from Plant Samples before LC-MS/MS.
Not available
S-EPMC10743447 | biostudies-literature
Proteomics3D-Printed Device for Consistent and High-Throughput Peptide Sample Clean-up Using StageTips
Mass spectrometry (MS) is a fundamental technology for the study of proteomics. A core technique for MS analysis is solid-phase extraction (SPE) for the removal of contaminants and sample concentration, and StageTips are one of the most commonly used devices used for SPE in modern proteomics workflo...
ORGANISM(S): Homo sapiens (Human) 
2019-03-21 | PXD009324 | Pride
3d-printed Stagetip 96-well 
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