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ProteomicsRapid High-pH Reverse Phase StageTip for Sensitive Small-Scale Membrane Proteomic Profiling
Membrane proteins are crucial targets for cancer biomarker discovery and drug development. However, in addition to the inherent challenges of hydrophobicity and low abundance, complete membrane proteome coverage of clinical specimen is usually hindered by the requirement of large amount of starting ...
ORGANISM(S): Rattus rattus (Black rat) Homo sapiens (Human) 
2015-11-16 | PXD001548 | Pride
High-ph reverse phase stagetip Membrane proteins Lung cancer 
ProteomicsMining Missing Membrane Proteins by High-pH Reverse Phase StageTip Fractionation and Validation by Multiple Reaction Monitoring Mass Spectrometry
Several significant efforts have been made in the past decade to map all human proteome. However, more than 3000 proteins are grouped as missing having no evidence of translation. Membrane protein annotation of the current missing proteins list showed 34%to be missing membrane proteins, which are ch...
ORGANISM(S): Homo sapiens (Human) 
2015-07-27 | PXD002224 | Pride
High-ph reverse phase Stagetip Missing proteins Lung cancer 
ProteomicsRapid High-pH Reverse Phase StageTip for Sensitive Small-Scale Membrane Proteomic Profiling
To increase the coverage of membrane protein identification,we developed high-pH reverse phase (Hp-RP) combined with stop-and-go extraction tip (StageTip) technique, as a fast (∼15 min.). Almost 5000 proteins (∼60% membrane proteins) can be identified in only 10 μg of membrane protein digests, showi...
ORGANISM(S): Homo Sapiens (human) 
2016-09-08 | PXD004904 | JPOST Repository
UnknownMining Missing Membrane Proteins by High-pH Reverse-Phase StageTip Fractionation and Multiple Reaction Monitoring Mass Spectrometry.
Not available
S-EPMC4761227 | biostudies-literature
ProteomicsSialo-glycoproteome of Human lung cancer cell by LC-MSMS
Alterations in protein glycosylation, especially the terminal sialylation, are closely correlated with physiological and pathological regulation. Due to the low ionization efficiency of sialo-glycopeptides and frequently observed dissociation of sialic acid residues during mass spectrometry analysis...
ORGANISM(S): Homo sapiens (Human) 
2022-02-25 | PXD010456 | Pride
Sialo-glycoproteome Glycoproteome Human lung cancer 
ProteomicsMetal ion-decorated ZIC-cHILIC StageTip Enables Simultaneous Profiling of N-Glycoproteome and Phosphoproteome in TKI resistant NSCLC cells
Cell membrane proteins are densely decorated with surface glycosylation and intracellular phosphorylation whose interplay determines the cell-cell communication and signaling cascades. However, their concomitant characterization remains extreme challenges due to complexity of glycan structures, low ...
ORGANISM(S): Homo sapiens (Human) 
2025-08-04 | PXD056077 | Pride
Phosphoproteome N-glycoproteome Zic-chilic 
OtherIn-depth proteomic analysis of mouse microglia using a combination of FASP and StageTip-based, high pH, reversed-phase fractionation.
Data from ProteomeXchange, PXD ID: PXD000168. Experiment: BV_2_High_pH_biological_1, file: 20121208_L_4.mzML. Published as part of Proteomics. 2013 Aug 14 . From the Abstract: {{i}} ... A characterization of microglia proteome would facilitate on the study of microglial functions in association wit...
ORGANISM(S): Mouse, Abelson_murine_leukemia_virus, Ectromelia_virus, Mouse_mammary_tumor_virus, Mouse_parvovirus_1, Mouse_parvovirus_2, Mouse_parvovirus_3, Mouse_parvovirus_4a, Mouse_parvovirus_5a, Murid_herpesvirus_1, Murid_herpesvirus_2, Murid_herpesvirus_4, Murine_adenovirus_3, Murine_adenovirus_a, Murine_hepatitis_virus_a59, Murine_hepatitis_virus_jhm, Murine_norovirus_1, Murine_osteosarcoma_virus, Murine_pneumotropic_virus, Murine_polyomavirus, Murine_type_c_retrovirus 
GPM11210021378 | GPMDB
Resource Reanalysis 
ProteomicsJPST000129-2 Stagetip fractionation for Phosphoproteome [Reanalysis: JPST000129]
Phosphoproteme analysis using C18-SCX stagetip fractionation. [Original project description]
ORGANISM(S): Homo Sapiens (human) 
2023-03-25 | RPXD034734 | JPOST Repository
ProteomicsJPST000129-1 Stagetip fractionation for Phosphoproteome [Reanalysis: JPST000129]
Phosphoproteme analysis using C18-SCX stagetip fractionation. [Original project description]
ORGANISM(S): Homo Sapiens (human) 
2023-03-25 | RPXD034733 | JPOST Repository
OtherProteomic analysis of mouse astrocytes and their secretome by a combination of FASP and StageTip-based, high pH, reversed-phase fractionation.
Data from ProteomeXchange, PXD ID: PXD000501. C8-D1A cells analyzed. Experiment: C8D1A_secretome_set1_2, file: 20130530_C8D1A_secretome_set1_F6_2.mzml. Published as part of Proteomics. 2014 Apr 17 . From the Abstract: {{i}} Astrocytes are the most abundant cells in the CNS, but their function remai...
ORGANISM(S): Mus_musculus_viruses, Mouse 
GPM32320000882 | GPMDB
Resource Reanalysis 
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