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MetabolomicsProtein-metabolite interactomics of carbohydrate metabolism reveal regulation of lactate dehydrogenase

Metabolic networks are interconnected and influence diverse cellular processes. The protein-metabolite interactions that mediate these networks are frequently low affinity and challenging to systematically discover. We developed mass spectrometry integrated with equilibrium dialysis for the disco...

2023-08-07 | MTBLS7237 | MetaboLights
MetabolomicsChanges in L-phenylalanine concentration reflect and predict response to anti-PD-1 treatment combined with chemotherapy in patients with non-small cell lung cancer
Chemotherapy combined with checkpoint blockade antibodies targeting programmed cell death protein (PD-1) has achieved remarkable success in non-small cell lung cancer (NSCLC). However, few patients benefit from long-term treatment. Therefore, biomarkers capable of guiding the optimal therapeutic sel...
2025-06-16 | MTBLS11232 | MetaboLights
MetabolomicsThe patatin-like protein PlpD forms novel structurally dynamic homodimers in the Pseudomonas aeruginosa outer membrane

Members of the Omp85 superfamily of outer membrane proteins (OMPs) found in Gram-negative bacteria, mitochondria and chloroplasts are characterized by a distinctive 16-stranded β-barrel transmembrane domain and at least one periplasmic POTRA domain. All previously studied Omp85 proteins promote c...

2025-12-08 | MTBLS9803 | MetaboLights
MetabolomicsIntegrating two chromatographies in a co-fractionation mass spectrometry approach improved protein-metabolite interactions (PMIs) mapping capabilities and revealed novel protein-ligand pairings in Escherichia coli.
The protein-metabolite interactome remains understudied. We previously developed a co-fractionation mass-spectrometry-based approach called PROMIS to address this gap. PROMIS relies on size separation chromatography of protein-metabolite complexes from lysates, followed by proteomics and metabolomic...
2025-04-25 | MTBLS9098 | MetaboLights
MetabolomicsThe Bardet-Biedl protein Bbs1 controls photoreceptor outer segment protein and lipid composition

Summary

Primary cilia are key sensory organelles whose dysfunction leads to ciliopathy disorders such as Bardet-Biedl syndrome (BBS). Retinal degeneration is common in ciliopathies, since the outer segments (OSs) of photoreceptors are highly specialized primary cilia. BBS...

2022-02-18 | MTBLS4013 | MetaboLights
MetabolomicsA targeted MRM-based lipidomic dataset of Tenebrio molitor larvae fed diets with different protein levels
Tenebrio molitor is increasingly used as a sustainable source of nutrients for food and feed production, yet their lipid metabolic responses to dietary protein remain poorly characterized at the molecular species level. Here, we report a targeted UPLC–MS/MS lipidomics dataset of Tenebrio molitor lar...
2026-05-20 | MTBLS14531 | MetaboLights
MetabolomicsBacteria encode their own post-mortem protein catabolism to enable nutrient recycling
For many organisms, death is a tangible, visible event. In bacteria, death can be a natural or an induced process 1. Death permits genes conferring a survival advantage to be selected in a bacterial population. Furthermore, biomolecule recycling from dead bacteria is crucial under nutrient limitatio...
2025-01-23 | MTBLS10044 | MetaboLights
MetabolomicsTranscript, protein and metabolite temporal dynamics in the CAM plant Agave
Already a proven mechanism for drought sustainability, crassulacean acid metabolism (CAM) is a specialized type of photosynthesis that maximizes water-use efficiency (WUE) via an inverse (compared to C3 and C4 photosynthesis-performing species) day/night pattern of stomatal closure/opening to shift ...
2017-10-13 | MTBLS363 | MetaboLights
MetabolomicsMonitoring recombinant protein expression in bacteria by rapid evaporative ionisation mass spectrometry

RATIONALE: There is increasing interest in methods of direct analysis mass spectrometry that bypass complex sample preparation steps.

METHODS: One of the most interesting new ionisation methods is rapid evaporative ionisation mass spectrometry (REIMS) in wh...

2022-01-28 | MTBLS957 | MetaboLights
Metabolomics1H NMR metabolomic and transcriptomic analyses reveal urinary metabolites as biomarker candidates in response to protein undernutrition in adult rats

BACKGROUND: Protein undernutrition is prevalent mainly among elderly people, and it could develop diseases such as sarcopenia. Urinary metabolites may become biomarkers reflecting protein undernutrition, but this has been insufficiently investigated.

OBJECTIVE:

2021-05-27 | MTBLS1452 | MetaboLights
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